Laboratory Test Information Guide

Details for Alkaline Phosphatase Isoenzymes, Serum

Test Name: Alkaline Phosphatase Isoenzymes, Serum
Alternate Name(s): Alkaline Phosphatase Fractionation
ALP Isoenzymes
Laboratory: Clinical Immunology
Specimen Type: Adult:
5 mL Gold top Vacutainer tube

Pediatric:
0-2 years: 0.5 mL Red or Gold top Microtainer
2-10 years: 3 mL Red top Vacutainer tube

Light Green (Li-Heparin) or Lavender (EDTA) top tubes are NOT acceptable
Collection Information: Minimum volume of serum required is 200 L.
Requisition: GENERAL LABORATORY REQUISITION
Test Schedule: Monday - Friday
0800-1600
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
10 days
Stat
N/A
 
Reference Range: Adult (20 50 years):

Liver 1: ≤ 64 U/L
Bone: ≤ 73 U/L
Liver 2: ≤ 8 U/L
Intestinal: ≤ 10 U/L
Effective Date: 2010-01-11
Revised Date: 2018-10-22
Critical Value:  
Interpretive Comments: 1. The reference values of the individual isoenzymes vary with age, sex, hormonal state (pregnancy, menopause, puberty) and medications.

2. In children, ALP is increased due to predominant bone isoenzymes.

3. Intestinal isoenzymes are absent in about 60% of normal subjects; when present they do not exceed 10 IU/L.

4. Our method does not differentiate Placental ALP from other bands.
Special Processing:  
Comments: If alkaline phosphatase isoenzymes are ordered and it has been ≤ 15 days since the collection date of the last sample run, the test will be cancelled. Please monitor using the total alkaline phosphatase result.
Method of Analysis: Sebia gel electrophoresis
Critical Information Required:  
Storage & Shipment: For short transit times (< 2 hours), ship at ambient temperature or on cold packs.
For transit times > 2 hours, freeze sample and ship with ice packs.
Store at 20 C.
System Codes: 
Cerner
ALPISO
 
Referred To:  

Questions? Comments? Contact Laura Gopaul