Laboratory Test Information Guide

Details for Calcitonin, Needle Rinse Fluid

Test Name: Calcitonin, Needle Rinse Fluid
Alternate Name(s): Thyrocalcitonin
Laboratory: Endocrinology
Specimen Type: Two samples:
The first is unused saline and serves as a blank.
The second is saline from the needle rinse, collected as described below.

Each sample should be collected in a plain, plastic, screw-top tube.
Collection Information: 1. Needle wash specimens for analysis should be collected in conjunction with cytology specimens. After each fine-needle aspiration biopsy (FNAB) has been collected and the material in the needle has been expelled onto a slide for cytologic analysis, attach the used FNAB needle to an empty syringe.
2. Withdraw between 0.10-0.25 mL of saline up through the needle until the saline starts to fill the hub of the needle or end of the syringe.
3. Expel this fluid back through the needle into a separate tube. This is the needle washing used for analysis.
4. Repeat steps 1-3 for each needle pass of the same biopsied site and empty into the same tube, accumulating a total of 0.5-1.5 mL of fluid to send to the laboratory. If more than one site is biopsied, each washing material should be submitted on a separate tube and under a different order number. The biopsied site of each specimen must be clearly identified.
5. Also collect 0.5-1.5 mL of saline that has not be used for needle rinse. This will be analyzed and used as a control since the assay has not been validated using saline as a sample type.
6. Deliver samples to the lab as soon as possible after collection.
Test Schedule: Monday Friday
Turnaround time from when specimen
is received in testing laboratory:
5 days
Reference Range: There is no reference interval available for this specimen type. Interpretation should be made based on comparison to the saline control and cytology results.
Effective Date: 2017-09-18
Revised Date:  
Critical Value:  
Interpretive Comments: Note: Testing method changed September 18, 2017.
Special Processing: Inspect specimen for visible blood or tissue contamination. If bloody, centrifuge specimen and transfer supernatant to a new plastic aliquot tube (5 mL standard tube). The supernatant, not the cellular material, is used for analysis. If specimen is clear, centrifugation is not necessary.

Samples should be frozen as soon as possible.
Comments: This test is only available at the request of Dr. Deric Morrison. Any other physicians ordering this test will require biochemist approval from Dr. Angela Rutledge at extension 77626.
Method of Analysis: Diasorin Chemiluminescence
Critical Information Required:  
Storage & Shipment: Store and ship frozen.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul