Laboratory Test Information Guide

Details for Chromosome Analysis, Bone Marrow/Blood Oncology Studies

Test Name: Chromosome Analysis, Bone Marrow/Blood Oncology Studies
Alternate Name(s): Chromosomes
Karyotype
Banding
G-Banding
Laboratory: Cytogenetics (VH)
Specimen Type: 1-2 mL of bone marrow in a 3 mL Sodium Heparin Vacutainer (see Collection Information)
Note: Bone Marrow is the preferred sample for chromosome studies

or

3-6 mL peripheral venous blood in a sterile, Sodium Heparin Vacutainer. If <3 mL is collected, it must be in a 3 mL Vacutainer to allow for appropriate sample to anticoagulant ratio.

Peripheral blood samples require >10% blasts for chromosome study
Collection Information: Specimens should be collected at room temperature (15-25șC) and sent STAT to the laboratory. Receipt of specimens is required the same day as collection. Do not freeze, heat, open or separate sample (must maintain sterility). Lithium heparin or EDTA specimens are unacceptable. All containers should contain at least two patient identifiers.
Requisition: CYTOGENETICS REQUISITION must include patient's name, address, Ontario Health Insurance Number, PIN (if applicable), originating location, Dr's full name and address, test requested, specimen type and pertinent clinical information
Test Schedule: As required
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
3 weeks
Stat
1 week
 
Reference Range: See final report
Effective Date:  
Revised Date: 2011-05-11
Critical Value: See final report
Interpretive Comments: Useful in diagnosing various types of leukemia’s and for other malignancies. Used to monitor bone marrow transplants.
Special Processing: Culture success is dependent on receipt of the sample immediately.
Comments: The Cytogenetics Lab is staffed from 0700-1700 (Monday-Friday), Ext. 78974 (office), or 75714 (lab).

For additional information please refer to the Molecular Diagnostics Laboratory.
Method of Analysis: Cell culture, Chromosome Karyotyping
Critical Information Required: Peripheral blood samples require >10% blasts for chromosome study.
Appropriate analysis requires all relevant and pertinent clinical information.
Culture success is dependent on receipt of the sample immediately.
A sodium heparin vacutainer is required (all other anticoagulants are unacceptable).
Storage & Shipment: Specimens should be shipped same day and stored at 15șC-25șC during transit. If unable to transport immediately, store at 4oC and transport at room temperature (15-25oC) within 24 hours.
System Codes: 
Cerner
 
 
Referred To:  

Questions? Comments? Contact Laura Gopaul