Laboratory Test Information Guide

Details for Complement Components

Test Name: Complement Components
Alternate Name(s): CCOMPG
Complement components group test: C1qrs, C5, C6, C7, C8, C9
(Each component may be ordered separately)
Laboratory: Clinical Immunology
Specimen Type: Age 10-Adult:
6 mL Red top Vacutainer tube and 5 mL Lavender top (K3EDTA) tube
Require 2 x 1 mL aliquots each of serum and plasma for send out

Pediatric:
0-2 yrs: 2 x 0.5 Red micropick and 2 x 0.5 Lavender micropick
Require 2 x 0.5 mL aliquots each of serum and plasma for send out

2-10 yrs: 2 x 4 mL Red top and 2 x 4 mL Lavender top
Require 2 x 1 mL aliquots each of serum and plasma for send out
Collection Information: Both plasma and serum are required.
Request CH50 (TCOM) on the same sample. (CH50 is performed by the Immunology Lab.)

Note that all requests will be screened first by a Total complement. Only if this screen is below the normal range will the complement component be sent out; if the screen is normal or high then the request will be cancelled.

Record collection time.
Mix the lavender top tube well after procurement.
Send the sample to the laboratory ASAP.

Mix the Lavender tube well after procurement. Centrifuge at room temperature or in a warm centrifuge as soon as possible after collection. Aliquot the plasma into two clean storage tubes and freeze as rapidly as possible.

Allow the filled Red top tube to clot at 22 - 37oC for 30 60 minutes. Centrifuge at room temperature or in a warm centrifuge. Aliquot into two clean storage tubes and freeze as soon as possible.
Requisition: GENERAL LABORATORY REQUISITION
Test Schedule: Referred out monthly.
Total complement CH50 is performed as a screen to determine the component assay is required.
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
40 days
Stat
N/A
 
Reference Range: 

C1q 83 to 125 mg/L
C1r 61 to 162 % of standard
C1s 59 to 297 % of standard
C5 55 to 113 mg/L
C6 28 to 69 mg/L
C7 35.3 to 96.5 mg/L
C8 49 to 106 mg/L
C9 33 to 95 mg/L
Effective Date: 2010-01-11
Revised Date:  
Critical Value:  
Interpretive Comments: Group test that includes, C1qrs, C5, C6, C7, C8, C9.

Not included in the group test: C1 esterase inhibitor, C2, C3, C4.

Total complement (TCOM, CH50) is a screen for classical pathway function.
The component assay is a quantitative evaluation of the concentration of each component but does not evaluate the functional activity of the component.

In vivo consumption or inherited complement deficiency may result in decreased levels of individual components.

The absence of a single component when the others are normal or elevated suggests genetic deficiency of the missing component.The absence or decreased levels of multiple components suggests consumption.
Special Processing: Centrifuge lavender tube (plasma) at room temperature as soon as possible after collection. Aliquot the plasma into 2 storage tubes and freeze as rapidly as possible.
Allow the filled red top tube to clot at 22-37oC for 30-60 minutes. Centrifuge at room temperature. Aliquot into storage tube and freeze as soon as possible. Store at -20oC (preferably -70o).

Aliquots:
For CH50-1 Aliquot (serum)
For CCOMPG- 2 Aliquots (plasma)
Comments: This test is available exclusively to SJHC/LHSC physicians.

CCOMPG is a group test that includes C1qrs, C5, C6, C7, C8, C9. The components can be ordered individually.

Total complement is assayed first to determine if the components evaluation is necessary. Only if the total complement is less than the lower limit of normal will the complement component be processed. Otherwise the component(s) is cancelled with the Comment: "Test not indicated."
Method of Analysis: RID
Critical Information Required:  
Storage & Shipment: LHSC (SRA to Immunology Laboratory) ship frozen on ice packs. Avoid freeze/thaw cycles.
System Codes: 
Cerner
CCOMPG
 
Referred To: Components referred to:

National Jewish Medical and Research Center
1400 Jackson St.,
Denver, Colorado
80206

Questions? Comments? Contact Laura Gopaul