Laboratory Test Information Guide

Details for Differential Leukocyte Count (Peripheral Blood)

Test Name: Differential Leukocyte Count (Peripheral Blood)
Alternate Name(s): White Blood Cell Differential
Lymphocytes
Monocytes
Neutrophils
Bands
Eosinophils
Basophils
Laboratory: Core
Specimen Type: Adult:
4 mL K2 EDTA (Lavender) Vacutainer tube

Pediatric Venous:
2 mL Paeds K2 EDTA (Lavender) Vacutainer

Pediatric (Capillary):
0.5 mL MAP K2 EDTA (Lavender) Microtube
0.5 mL K2 EDTA (Lavender) Microtube
Collection Information: A differential may be added to a CBC (complete blood count) order up to 8 hours from collection of the sample. Red cell morphological assessment will not be completed if the sample is > 4 hours old.
Requisition: GENERAL LABORATORY REQUISITION
Test Schedule: As required
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
> 4 hours
Stat
2 hours
 
Reference Range: Lymphocytes:
18 yrs.:
1.0-4.0 x 109/L
10-17 yrs.:
1.5-4.0 x 109/L
2-9 yrs.:
2.0-8.0 x 109/L
1-23 months:
4.0-10.5 x 109/L
Newborn-30 days:
2.0-17.0 x 109/L

Monocytes:
>18 yrs.:
0.2-0.8 x 109/L
10-17 yrs.:
0.2-0.8 x 109/L
2-9 yrs.:
0.2-0.8 x 109/L
1-23 months:
0.2-1.5 x 109/L
Newborn-30 days:
0.2-3.1 x 109/L

Neutrophils:
>18 years:
2.0-7.5 x 109/L
Critical Low: 0.6 x 109/L
10-17 yrs.:
1.5-8.0 x 109/L
2-9 yrs.:
1.5-8.5 x 109/L
1-23 months:
1.5-8.5 x 109/L
Newborn-30 days:
6.0-26.0 x 109/L

Bands:
>18 yrs.:
0-0.5 x 109/L
10-17 yrs.
0-1.0 x 109/L
2-9 yrs.
0-1.0 x 109/L
1-23 months:
0-1.0 x 109/L
Newborn-30 days:
0-3.5 x 109/L

Eosinophils:
>18 yrs.:
0.0-0.4 x 109/L
10-17 yrs.
0-0.4 x 109/L
2-9 yrs.:
0-0.5 x 109/L
1-23 months:
0-0.7 x 109/L
Newborn-30 days:
0-1.5 x 109/L

Basophils:
>18 yrs.:
0-0.1 x 109/L
10-17 yrs.:
0-0.1 x 109/L
2-9 yrs.:
0-0.1 x 109/L
1-23 months:
0-0.1 x 109/L
Newborn-30 days:
0-0.4 x 10 9/L






Effective Date: 2007-07-18
Revised Date: 2016-06-09
Critical Value: The presence of blasts and/or an absolute neutrophil count ≤0.5 x 109/L
Interpretive Comments: Normal ranges were adopted through consensus by the three London teaching hospitals (VH, UH, SJH). Adult reference ranges have been validated on current instrumentation by testing of normal donors.
Special Processing:  
Comments:  
Method of Analysis: Automated differential generated by Hematology analyzer, based on characteristic light scattering properties. Confirmed with microscopic examination when required.
Critical Information Required:  
Storage & Shipment:  
System Codes: 
Cerner
CBCD
 
Referred To:  

Questions? Comments? Contact Laura Gopaul