Laboratory Test Information Guide

Details for HLA ABC Typing

Test Name: HLA ABC Typing
Alternate Name(s): ABC Tissue Typing
HLA Class I Typing
Laboratory: Transplant (UH)
Specimen Type: 4 x 6 mL Green (Sodium Heparin) top Vacutainer tubes without gel separator
This is the preferred specimen for patients with normal leukocytes

or

2 x 4 mL Lavender (EDTA) top Vacutainer tubes
-Use when leukocyte irregularities are possible (all potential bone marrow / stem cell recipients or HLA matched platelet recipients) or if the transit time is expected to exceed 24 hours.

Note:
-Increase collection volumes if leukocyte count is low
-Smaller volumes apply for pediatric patients

Pediatric patients:

1 x 6 mL Dark Green (Sodium Heparin) top Vactuainer tubes without gel separator
or
1 x 4 mL Lavender (EDTA) top Vacutainer tube when leukocyte irregularities are possible (all potential bone marrow / stem cell recipients or HLA matched platelet recipients) or if the transit time is expected to exceed 24 hours

Contact the laboratory if these volumes are not possible (519-663-3320)
Collection Information: Samples that are being shipped into the laboratory should be drawn and shipped on Mondays, Tuesdays and Wednesdays only.

Draw samples prior to treatment if chemotherapy is being considered.
Requisition: TRANSPLANT LABORATORY REQUISITION
Test Schedule: Monday to Friday
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
7-21 days
Stat
Same day if the sample is received in the Transplant Lab before 0830 (M-F except Stat Holidays)
 
Reference Range: See report
Effective Date: 2006-07-07
Revised Date: 2012-10-15
Critical Value: N/A
Interpretive Comments: The report will list the Class I HLA antigens or alleles identified for the patient.
Special Processing: HLA Disease Association
HLA Transfusion Typing (ABC)


This test is also a component of the following Care Sets:
HLA Typing (ABCD)
HLA Assessment – Non Renal Recipient
HLA Assessment – Renal Recipient
HLA BM/SC Histocompatibility – Donor
HLA BM/SC Histocompatibility – Recipient
HLA Confirmatory Typing – Donor
HLA Confirmatory Typing – Recipient
HLA Workup Deceased Donor
HLA Workup Living Donor – Initial
Comments: Bone Marrow / Stem Cell donors- include the recipient’s name, relationship, physician and transplant centre. Testing is only performed for patients involved in the London Transplant Program. Unrelated marrow / stem cell donors are not normally tested in this laboratory. Please contact the laboratory (519-663-3320) if other situations apply to your patient.

Disease Association HLA typings- Indicate the disease under investigation and the antigen of interest. This test is only appropriate for diseases associated with HLA ABC / Class I antigens. See HLA DR/DQ Tissue Typing for diseases associated with HLA DR/DQ or Class II antigens.
Method of Analysis: Microlymphocytotoxicity or PCR-SSP
Critical Information Required: Family investigations (i.e. bone marrow/HSC transplant) must include the identity of the potential recipient and the relation of all potential donors.
Storage & Shipment: Keep heparinized blood at room temperature and deliver within 24 hours.

Keep EDTA blood at room temperature and deliver within 72 hours.
System Codes: 
Cerner
See Special Processing
 
Referred To:  

Questions? Comments? Contact Laura Gopaul