Laboratory Test Information Guide

Details for HLA CDC Crossmatch for Organ Transplant (Donor Requirements for a Deceased Donor Situation)

Test Name: HLA CDC Crossmatch for Organ Transplant (Donor Requirements for a Deceased Donor Situation)
Alternate Name(s):  
Laboratory: Transplant (UH)
Specimen Type: Donor blood for a workup involving a kidney or kidney/pancreas (+/- other organs) transplant:
10 x 8.5 mL ACD (Solution A) (yellow tops)*
1 x 4 mL Lavender EDTA top Vacutainer tube

Donor blood for a workup involving a heart transplant only:
8 x 8.5 ml. ACD (Solution A) (yellow tops)*
1 x 4 mL Lavender EDTA top Vacutainer tube

Donor blood for a workup involving a liver transplant only:
1 x 4 mL Lavender EDTA top Vacutainer tube
*Substitute 14 x 6 mL Sodium Heparin (dark green tops, no separator gel) top Vacutainer tubes if ACD tubes are not available.

NOTE: keep at blood at room temperature

NOTE: the blood requirements listed above include samples that will be used for the HLA Class I and HLA Class II typing of the donor.

NOTE: additional blood samples are required for testing unrelated to HLA. i.e. ABO grouping, viral serology etc. Contact the donor coordinator or see the insert in the donor kits (see collection information below) for details.

NOTE: smaller volumes apply for pediatric kidney donors.
5 x 8.5 mL ACD (Solution A) (yellow tops)**
1 x 4 mL Lavender EDTA top Vacutainer tube

**Substitute 7 x 6 mL Sodium Heparin (dark green tops, no separator gel) if ACD tubes are not available.

NOTE: a small section of donor spleen (approx. 2 cm. X 2 cm.) or several lymph nodes are required at the time of organ retrieval.
Collection Information: Pre-packaged “kits” which include tubes and requisitions required for a deceased donor crossmatch are available from the Transplant Laboratory. Call 519-663-3320 for details.
Some of the tubes in these kits are for tests performed in other laboratories i.e. ABO grouping, viral serology etc. The SRA staff will distribute the samples to the appropriate areas.
Requisition: Transplant Immunology Including HLA Antigens
Test Schedule: As required
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
N/A
Stat
8 to 12 hours
 
Reference Range: See report
Effective Date: 2006-07-07
Revised Date: 2012-10-15
Critical Value: N/A
Interpretive Comments: A positive crossmatch indicates the presence of anti-donor antibodies.
Special Processing: Deceased donor crossmatch situations should be considered STAT and sample delivery should be handled accordingly.

SRA staff: please page the donor coordinator on-call when donor blood arrives. The donor coordinator will then page the Transplant Laboratory technologist on-call.
Comments: A deceased donor situation must be coordinated by the TGLN and/or the local donor coordinators.

TGLN: 1-877-363-8456
Local Donor Coordinators: contact LHSC switchboard @ 519-685-8500
Method of Analysis: Microlymphocytotoxicity using techniques of enhanced sensitivity (AHG &/or extended incubation)
Critical Information Required: Indicate the classification of the donor, if possible i.e. Standard Criteria (SCD), Extended Criteria (ECD), Donation following Cardiac Death (DCD).

Indicate the organs being considered for transplant, if possible.
Storage & Shipment: Transport and store blood at room temperature.
Transport and store spleen refrigerated.

See Special Processing information above.
System Codes: 
Cerner
HLA Workup Deceased Donor
 
Referred To:  

Questions? Comments? Contact Laura Gopaul