Laboratory Test Information Guide

Details for HLA CDC Crossmatch for Organ Transplant (Donor and Recipient Requirements for a Living Donor Situation)

Test Name: HLA CDC Crossmatch for Organ Transplant (Donor and Recipient Requirements for a Living Donor Situation)
Alternate Name(s):  
Laboratory: Transplant (UH)
Specimen Type: Donor Requirements-Initial Crossmatch*:
14 x 6 mL Sodium Heparin (dark green tops, no separator gel)
1 x 4 mL Lavender EDTA top Vacutainer tube

*The blood requirements listed above include samples that will be used for the HLA Class I and HLA Class II typing of the donor plus the flow cytometry crossmatch, if required.

Donor Requirements-Additional/Final Crossmatch**:
14 x 6 mL Sodium Heparin (dark green tops, no separator gel

**The blood requirements listed above include samples that will be used for the flow cytometry crossmatch, if required.

Recipient Requirements (all crossmatches):
1 x 6 mL Red top Vacutainer tube
Draw by venipuncture and pre-dialysis (if applicable) to avoid contamination with anticoagulants.

NOTE: smaller volumes apply for paediatric patients. Contact the laboratory for details (519-663-3320).
Collection Information: Living donor crossmatches are performed only for patients in the London Transplant Program and must be booked in advance by calling the laboratory. (519-663-3320)

The living donors are bled at the UH LTC early in the morning and samples are delivered immediately to the Transplant Laboratory (room temperature) for same day testing. Shipped samples are not accepted.

The recipient blood (serum) can be shipped but it must be in the laboratory before the donor blood arrives.


Cerner Codes:
Donor Tests:
HLA Workup Living Donor Initial
HLA Workup Living Donor Additional/ Final

Recipient Tests:
HLA serum for crossmatch LD Recipient
Requisition: Transplant Immunology Including HLA Antigens
Test Schedule: Monday to Thursday
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
7-21 days
Stat
24-48 hours
 
Reference Range:  
Effective Date: 2011-03-15
Revised Date: 2012-10-15
Critical Value:  
Interpretive Comments: A positive crossmatch indicates the presence of anti-donor antibodies.
Special Processing:  
Comments: Living donor transplant workups, including the crossmatching, are coordinated by the Living Donor Transplant Coordinator who can be contacted via the LHSC switchboard (519-685-8500).
Method of Analysis: Microlymphocytotoxicity using methods of enhanced sensitivity (AHG &/or extended incubation)
Critical Information Required:  
Storage & Shipment: Donor samples are drawn in the Lab Test Centre (UH) and delivered immediately to the Transplant Laboratory at room temperature.

Recipient sera samples that are drawn in the Lab Test Centre (UH) are to be delivered immediately to the Transplant Laboratory. Those that are to be shipped and received within 24 hours are to be stored and shipped refrigerated. Recipient samples that are delayed beyond 24 hours are to have the serum separated, frozen and shipped on dry ice (or frozen ice packs, if dry ice is not available).
System Codes: 
Cerner
See Collection Information
 
Referred To:  

Questions? Comments? Contact Laura Gopaul