Laboratory Test Information Guide

Details for Heparin Assay

Test Name: Heparin Assay
Alternate Name(s): Anti Xa Assay
Laboratory: Hemostasis and Thrombosis Laboratory (Victoria Hospital)
Specimen Type: 1 x 2.7 mL Blue (3.2% Na Citrate) Vacutainer tube

Pediatric:
0-2 years: 1.8 mL Sodium Citrate Coagulation tube:
Contact HAT lab ext. 52526 for number of tubes required prior to sampling
Collection Information: All test requests, regardless of whether the patient is an adult or pediatric, must be authorized by a Hematologist (listed in the Comments section below).
Requisition: GENERAL LABORATORY REQUISITION
Test Schedule: As authorized, Monday to Friday 0800-1600, unless otherwise requested by a Hematologist.
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
24 hours
Stat
4 hours
 
Reference Range: See report
Effective Date: 2009-06-10
Revised Date: 2019-04-10
Critical Value:  
Interpretive Comments:  
Special Processing: Blue (Sodium Citrate) top tubes should be centrifuged within 1 hour of collection.

The blood specimen must be double centrifuged to prepare platelet free plasma.

Centrifuge the primary tube or tubes for 10 minutes at 3000 rpm, aliquot and re-spin for an additional 10 minutes at 3000 rpm at room temperature (18 to 20 degrees Celsius). Ensure the temperature does not exceed 20 degrees Celsius.

The double-centrifuged plasma is aliquoted into plastic tubes labelled with the appropriate Cerner generated aliquot label and frozen immediately at -20 degrees Celsius or below.

SRA Processing

UH/SJH All shifts:

Process the samples as per the procedure above. Place samples/aliquot labels with transfer list in the SPEC COAG Bin in the -20C freezer. All samples to be sent to VH SRA the following morning. Upon arrival VH SRA will place the samples in the -20 bin. The HAT staff will pick up the samples.

VH 0700-1500 shift:

Place the samples/aliquot labels in the pneumatic tube and send to the 10th floor HAT Lab at VH. (No transfer list is required). Call HAT Lab at ext. 52526 to let them know a sample is coming.

VH after 1500:

Process the samples as per the procedure above. Place samples/aliquot labels in the SPEC COAG Bin in the -20C freezer. All samples will be picked up the following morning by the HAT staff.

VH Referred-In samples:
When a sample is received from an outside healthcare institution, please order SPCOAGRI, put a small accession label on the requisition, then place the frozen samples and labels in the SPEC COAG Bin in the -20 freezer. Please send the requisition with the accession number on it, to the 10th floor. These samples will be held until the next morning and picked up by the HAT Lab staff. If the HAT Lab staff require a sample earlier then the next morning, they will come down and get it. Call the HAT Lab at ext. 52526 to let them know the requisition is arriving.
Comments: Please direct any questions or concerns to:
Michael Keeney
Coordinator-Hematology & Flow Cytometry
519-685-8500 x 52187
Pager: 17716

All test requests, regardless of whether the patient is an adult or pediatric, must be authorized by a Hematologist (names and pagers listed below):

Dr. Michael Kovacs
Pager: 15182

Dr. Alejandro Lazo-Langner
Pager: 18970

Authorization of pediatric testing may be provided by:

Dr. Lawrence Jardine
Pager: 18392

Dr. Elizabeth Cairney
Pager: 15586

Dr. Shayna Zelcer
Pager: 17582
Method of Analysis: Chromogenic
Critical Information Required: The type of heparin being administered must be indicated.
Storage & Shipment: Blue (Na Citrate) top specimens should be centrifuged within 1 hour from the time of specimen collection and either tested immediately or plasma should be removed from cells and frozen.

Specimens may be kept for up to two weeks at 20 degrees Celsius or up to six months at 70 degrees Celsius.

Plasma aliquots, which require transportation, must be packaged appropriately to ensure samples remain frozen.

Once received by the Sample Receiving Department at Victoria Hospital, samples are to be stored at 70 degrees Celsius within 15 minutes.
System Codes: 
Cerner
HEPA
 
Referred To:  

Questions? Comments? Contact Laura Gopaul