Laboratory Test Information Guide

Details for Immunofixation Electrophoresis, Serum

Test Name: Immunofixation Electrophoresis, Serum
Alternate Name(s): IFE
IFE Serum
IFES
Light Chains
Laboratory: Clinical Immunology
Specimen Type: 5 mL Gold top Vacutainer tube

Light Green (Li-Heparin) or Lavender (EDTA) top tubes are NOT acceptable
Collection Information: Minimum volume of serum required is 2 mL.
Requisition: GENERAL LABORATORY REQUISITION
Test Schedule: Monday - Friday
0800-1600
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
10 days
Stat
N/A
 
Reference Range: Interpretative analysis
Effective Date: 2009-02-27
Revised Date: 2019-03-14
Critical Value:  
Interpretive Comments:  
Special Processing:  
Comments: Protein electrophoresis (PEL) is always performed first. The PEL result will determine if IFE is performed. IFE is performed when an abnormal globulin band is detected on PEL that has not been identified previously by IFE. If the band has been identified previously by IFE and the PEL pattern has not changed significantly, the IFE will not be repeated for ≥ 4 years.

IFE uses specific antisera to identify monoclonal immunoglobulins.
Method of Analysis: Sebia immunofixation electrophoresis
Critical Information Required:  
Storage & Shipment: For short transit times (< 2 hours), ship at ambient temperature.
For transit times > 2 hours, ship with ice packs.
Store at 2 8 C pre-analysis and 20 C post-analysis.
System Codes: 
Cerner
PELS
 
Referred To:  

Questions? Comments? Contact Laura Gopaul