Laboratory Test Information Guide

Details for Protein Electrophoresis, Serum

Test Name: Protein Electrophoresis, Serum
Alternate Name(s): PELS
Monoclonal Protein Screen
SPE
Serum Protein Electrophoresis
Laboratory: Clinical Immunology
Specimen Type: 5 mL Gold top Vacutainer tube

Light Green (Li-Heparin) or Lavender (EDTA) top tubes are NOT acceptable
Collection Information: If possible, please avoid collecting blood during dialysis or from patients treated with heparin. Blood collected in the presence of heparin may cause the appearance of a fibrinogen band on electrophoresis, which will require further processing to distinguish from a monoclonal protein. If not possible to avoid heparin contamination, please call the Clinical Immunology Laboratory at x33097 to inform the laboratory that the patient has received heparin treatment.

Minimum volume of serum required is 2 mL.

Grossly hemolyzed samples are not acceptable.
Requisition: GENERAL LABORATORY REQUISITION
Test Schedule: Monday - Friday
0800-1600
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
5 days
Stat
N/A
 
Reference Range: Albumin: 35.7 54.9 g/L
Alpha 1: 1.9 4.1 g/L
Alpha 2: 4.5 9.8 g/L
Beta 1: 3.0 6.0 g/L
Beta 2: 2.0 5.4 g/L
Gamma: 7.1 15.6 g/L
Effective Date: 2010-01-25
Revised Date: 2019-03-14
Critical Value:  
Interpretive Comments: Please note: new method as of October 31, 2018.

Abnormal bands are identified by immunofixation electrophoresis.
Special Processing:  
Comments: Based on consultation with the main users of the test, it has been decided to limit the collection frequency to ≥ 3 weeks for repeat testing. If serum protein electrophoresis is ordered and it has been <20 days since the collection date of the last sample run, the test will be cancelled.
Method of Analysis: Sebia capillary electrophoresis
Critical Information Required:  
Storage & Shipment: For short transit times (< 2 hours), ship at ambient temperature.
For transit times > 2 hours, ship with ice packs.
Store at 2 8 C pre-analysis and 20 C post-analysis.
System Codes: 
Cerner
PELS
 
Referred To:  

Questions? Comments? Contact Laura Gopaul