Laboratory Test Information Guide

Details for Sexing (for medical indications)

Test Name: Sexing (for medical indications)
Alternate Name(s): Sex Determination
Y-marker
Laboratory: Molecular Diagnostics
Specimen Type: Whole blood-1 x 4 mL Lavender EDTA top Vacutainer tube
Collection Information: Blood samples must be maintained at room temperature.
Requisition: MOLECULAR DIAGNOSTIC REQUISITION
Test Schedule: As Required
Monday - Friday
0800 - 1600 h
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
3 months
Stat
 
 
Reference Range: See report
Effective Date:  
Revised Date:  
Critical Value: N/A
Interpretive Comments: This test will be available for medical indications only, usually to aid in linkage analysis or to clarify prenatal diagnosis and must be referred from the Genetics Clinic.

The tooth enamel gene, amelogenin, is encoded on the short arm of the X chromosome1. The Y chromosome, however, bears an amelogenin-like sequence which is shorter by 177bp than that on the X chromosome. Knowing this, it is possible to determine tissue sex status, by PCR amplification followed by sizing with gel electrophoresis, of a fragment of this gene with specific oligonucletide primers2.

References

1. Lau, E.C., et al. Genomics (1989) 4: 162-168
2. Nakahori Y., et al. A.J.M.G.(1991) 39: 472-473
Special Processing:  
Comments: For more information click on:
MOLECULAR DIAGNOSTIC LABORATORY
Method of Analysis: PCR
Critical Information Required: Pedigree required.
Storage & Shipment: Must be received in testing laboratory within 5 days of collection, shipped at room temperature by courier/overnight delivery.
System Codes: 
Cerner
 
 
Referred To:  

Questions? Comments? Contact Laura Gopaul