Laboratory Test Information Guide

Details for Urine Culture (Urine C & S)

Test Name: Urine Culture (Urine C & S)
Alternate Name(s):  
Laboratory: Microbiology (VH)
Specimen Type: Urine
OR
Urine collected in the O.R.(Cytoscopy, kidney nephrostomy, suprapubic aspirate)
Collection Information: This sample type cannot be processed as a STAT request. Samples are handled in a priority sequence and results are reported as they become available.

Submit using B-D Vacutainer urine collection and transport system for Microbiology which consists of:


- Specimen cup with blue lid and integral sampling device for sample collection

- Grey top Vacutainer containing preservative for transport of sample to Microbiology Lab


This urine collection/transport system is for C & S (bacterial or fungal) only.

You must obtain at least 4 mL of urine in order to use this collection/transport system.
Samples obtained with less than 4 mL volume should be sent in sterile container (without preservative).


Sample collected for Acid Fast Bacilli or Legionella antigen detection or Ova and Parasite investigation are to be sent in a sterile container.
Samples collected for Chlamydia/Neisseria gonorrhoeae must be collected in the Genprobe Aptima collections system.
Please see specific orderables for specimen collection information.

Clean catch or midstream sample:
Send the first morning voided urine. To use collection cup, remove blue lid and place on counter with straw facing upward. Do not contaminate inner surface of lid or straw.


Female - Thoroughly clean the urethral opening and vaginal vestibule area with soapy water, then rinse or use a 40% Benzalkonium Chloride Towelette.
Hold the labia apart during voiding.
Allow a few millilitres of urine to pass. Do not stop the flow.
Collect the midstream portion in specimen cup.

Male - Cleanse the penis, retract the foreskin (if not circumcised), and wash with soapy water, then rinse or use a 40% Benzalkonium Chloride Towelette.
Keeping the foreskin retracted, allow a few millilitres of urine to pass. Do not stop the flow. Collect the midstream portion in specimen cup.

Ileal Conduit Urine

Remove the external urinary appliance, and discard the urine within.
Gently swab and clean the stomal opening with a 70% alcohol pad and then with an iodine solution.
Using sterile technique, insert a double catheter into the stoma.
Catheterize the ileal conduit to a depth beyond the fascial level.
Put the connector end of the catheter into the specimen cup and allow urine to flow by gravity.
Remove catheter and replace collection bag.
Remove excess iodine with 70% alcohol after procedure.

Catheter Urine (In/Out)

Prior to catheterization, the patient should force fluids until the bladder is full.
Clean the urethral opening (and in females, the vaginal vestibule) with soap, then rinse or use a 40% Benzalkonium Chloride Towelette.
Using sterile technique, pass a catheter into the bladder.
Collect the initial 15-30 mL of urine and discard it from the mouth of the catheter.
Collect sample in specimen cup.


Urine collected in the specimen cup must be transferred to a grey top Vacutainer tube for transport to lab for culture.
Replace lid on specimen cup and tighten to secure.
Peel back yellow label on specimen cup lid to expose sampling device.
Place grey top Vacutainer into sampling cavity and allow tube to fill until vacuum is exhausted.
Remove tube and shake to mix urine with preservative.

Catheter (indwelling)

Locate the urine sample port on the collection bag tubing.
Occlude tubing below port to create a small resevoir of urine.
Clean sample port with 70% alcohol.
Allow to dry. Insert blunt cannula/Vacutainer holder assembly into sample port.
Attach C & S grey top Vacutainer and allow tube to fill until vacuum is exhausted (minimum of 4 mL).
Remove tube and shake to mix urine with preservative.


O.R. Urines/Nephrostomy Urine Insertion (Interventional Radiology)

Collect urine in a sterile container. Collect in anaerobic transport media if anaerobes are requested.

Label the container/tube containing sample.

Urine collected from a urinal or bedpan is not acceptable.
Requisition: MICROBIOLOGY REQUISITION
Test Schedule: Daily
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
1-2 days
Stat
 
 
Reference Range:  
Effective Date:  
Revised Date: 2017-06-14
Critical Value:  
Interpretive Comments:  
Special Processing:  
Comments: In/Out Catheter and O.R. Urines are processed using a 10 ÁL inoculum which is reflected in the colony count.
Method of Analysis: Culture
Critical Information Required:  
Storage & Shipment: Refrigerate unpreserved samples.

Preserved specimens must be stored at room temperature.

The specimen must be received within 24 hours of collection.
System Codes: 
Cerner
URNC
 
Referred To:  

Questions? Comments? Contact Laura Gopaul