Laboratory Test Information Guide

Details for ACTH Stimulation Test, Plasma/Serum

Test Name: ACTH Stimulation Test, Plasma/Serum
Alternate Name(s): Cosyntropin Test
Laboratory: Core
Specimen Type: FOR ACTH TESTING:
Adult:

4 mL Lavender top (EDTA) Vacutainer tube

Pediatric:
0-2 years: 0.5 mL Lavender top (EDTA) Microtainer
2-10 years: 2 mL Lavender top (EDTA) Vacutainer tube
Red, Gold, or Light Green (Li-heparin) top tubes are NOT acceptable.

FOR CORTISOL TESTING:
Adult:

4.5 mL Light Green top (Li-Heparin) Vacutainer tube

Pediatric:
0-2 years: 0.5 mL Light Green top (Li-Heparin) Microtainer
2-10 years: 3 mL Light Green top (Li-Heparin) Vacutainer tube

Red, Gold, or Lavender (EDTA) top tubes are also acceptable

FOR TESTING OF OTHER ANALYTES:
Please consult the Laboratory Test Information Guide entries for those analytes to determine the appropriate specimen type.
Collection Information: FOR ACTH TESTING:
Pre-chill Vacutainer, place on ice immediately after blood collection, and deliver to lab immediately.

Minimum volume of plasma required is 700 L for adult samples or 200 L for pediatric samples.

FOR CORTISOL TESTING:
Minimum volume of plasma or serum required is 700 L for adult samples or 200 L for pediatric samples.

FOR TESTING OF OTHER ANALYTES:
Please consult the Laboratory Test Information Guide entries for those analytes to determine the appropriate collection information.
Requisition: Choose from (.ACTH0MST, .CORM30MST, .CORM15MST,.COR0MST, .COR15MST, .COR30MST, .COR45MST, .COR60MST, .COR75MST, .COR90MST, .COR105MST, .COR120MST, .COR180MST, .COR240MST) as appropriate and also include other analytes as appropriate
Test Schedule: For cortisol testing, as required.

For ACTH testing, Tuesday afternoons.
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
4 hours for cortisol; 5 days for ACTH
Stat
 
 
Reference Range: Interpretation of this test is based on the clinical circumstances, other medications and the formulation of ACTH used for this stimulation challenge.
Effective Date: 2019-12-09
Revised Date:  
Critical Value:  
Interpretive Comments:  
Special Processing: FOR ACTH TESTING:
Use a refrigerated centrifuge to separate plasma. Analyze samples immediately or freeze them at -20 C.
Comments: Biotin may interfere with the cortisol and ACTH tests. Samples should not be taken from patients receiving high biotin doses (i.e. > 5 mg/day) until at least 8 hours after the last biotin administration.

Oral contraceptives, pregnancy, or estrogen therapy cause elevated plasma cortisol levels due to an increase in binding proteins.

Patients suffering from 21-hydroxylase deficiency exhibit elevated 21-deoxycortisol levels and this can cause falsely elevated cortisol results.
Method of Analysis: Roche Electrochemiluminescence
Critical Information Required:  
Storage & Shipment: FOR ACTH TESTING:
Store and ship plasma frozen.
System Codes: 
Cerner
See above
 
Referred To:  

Questions? Comments? Contact Laura Gopaul