Laboratory Test Information Guide

Details for ANCA

Test Name: ANCA
Alternate Name(s): P-ANCA / Anti-MPO (anti-myeloperoxidase antibody)
C-ANCA / Anti-PR3 (anti-proteinase antibody)
Laboratory: Clinical Immunology
Specimen Type: 5 mL Gold top Vacutainer tube or 6 mL Red top EDTA, heparin or citrate plasma tube

Pediatric:
0-2 years: Red 1.0 pk.
2-10 years: 2 mL Red top
Collection Information: Minimum volume of serum required is 0.5 mL.
Requisition: GENERAL LABORATORY REQUISITION
Test Schedule: Wednesday and Friday

For STAT samples:
Availability of STAT testing is 07:00-15:00 Monday to Friday. Saturday, Sunday & holiday requests will be processed the next business day.
STAT testing must be pre-arranged with the Immunology Lab by calling x35541. See “Critical Information Required” section prior to calling.
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
3 days
Stat
See "Test schedule" section
 
Reference Range: Negative: : ≤19 RU/mL
Effective Date: 2010-12-09
Revised Date: 2018-01-15
Critical Value:  
Interpretive Comments: cANCA and pANCA are two fluorescence patterns that are associated with a number of antibodies. Our current methodology of ELISA detects anti MPO (a major antibody for pANCA)and antiPR3(a major antibody for cANCA)only.

ANCA testing is mainly indicated in patients with a tentative or a definite diagnosis of primary SVV (small vessel vasculitis).

C-ANCA/PR-3 antibodies prevail in patients with Wegener’s granulomatosis (80-95% of the patients).

P-ANCA/MPO antibodies prevail in patients with microscopic polyangiitis, Churg-Strauss syndrome and renal-limited SVV etc.
Special Processing:  
Comments: A single autoantibody test is not diagnostic and should not be used to determine course of treatment. The test result must be evaluated with consideration of clinical presentation, patient history and other laboratory tests.
Method of Analysis: Euroimmun Analyzer I-ELISA
Critical Information Required: STAT testing is for first diagnosis purposes only: patient monitoring will not be considered STAT.

STAT testing includes MPO, PR3 and GBM.

For STAT testing of MPO, PR3 and GBM a diagnosis of Microscopic polyangiitis, Wegener’s granulomatosis, Goodpasture syndrome or admitting symptoms of acute kidney failure or pulmonary distress must be provided for testing to commence.
Storage & Shipment: Intercampus – Ship via routine or stat specimen transport.

Serum can be stored for up to 4 days at 2 – 8oC. For longer storage, store at -20 oC.

Intercity – Freeze and store serum samples at –20oC . Ship on ice packs. Avoid freeze/thaw cycles.
System Codes: 
Cerner
ANCA
 
Referred To:  

Questions? Comments? Contact Laura Gopaul