Laboratory Test Information Guide

Details for Acetoacetate + Beta Hydroxybutyrate, Biochemical Genetics Lab

Test Name: Acetoacetate + Beta Hydroxybutyrate, Biochemical Genetics Lab
Alternate Name(s):  
Laboratory: Biochemical Genetics
Specimen Type: 6 mL Green (Sodium or Lithium Heparin) top Vacutainer tube

0-2 yrs: 2 x 0.5 mL Green top2-10 yrs: 3 mL Green top
Collection Information: Volume of blood required is 0.5 mL.

Collect blood, place on ice and send immediately to the Core Lab. Specimens not received on ice will not be processed.

Blood must be added to PCA immediately after collection in order to minimize degradation of metabolites.
Test Schedule: As required
Turnaround time from when specimen
is received in testing laboratory:
Reference Range: Acetoacetate:
0.02-0.08 mmol/L

0.06-0.17 mmol/L
Effective Date: 2008-06-10
Revised Date: 2020-06-05
Critical Value:  
Interpretive Comments:  
Special Processing: 1. Pipette 0.5 mL of freshly drawn whole blood from the Green top tube (on ice) into cold acetoacetate tube containing 1.0 mL of 8% perchloric acid (PCA).
2. Mix and spin in cold centrifuge.
3. Aliquot supernatant into clean transport tube.
4. Re-spin in cold centrifuge and remove supernatant into another clean transport tube.
5. Freeze immediately at -20°C.
6. Send frozen sample to Biochemical Genetics Laboratory
Comments: To be ordered for investigating biochemical genetics and metabolic disorders only. For all other requests refer to “Beta Hydroxybutyrate, Plasma/Serum” (test performed in the Core Lab).
Method of Analysis:  
Critical Information Required:  
Storage & Shipment:  
System Codes: 
Referred To: Biochemical Genetics Laboratory
London Health Sciences Centre
Victoria Hospital
Room B10-217
800 Commissioners Road East
London, Ontario
N6A 5W9

Joanne ext. 71560
Specimen Receiving ext. 71561
Fax: 519-858-1063

Questions? Comments? Contact Laura Gopaul