Laboratory Test Information Guide

Details for Adrenocorticotropic Hormone, Plasma

Test Name: Adrenocorticotropic Hormone, Plasma
Alternate Name(s): ACTH
Laboratory: Core
Specimen Type: Adult:
4 mL Lavender top (EDTA) Vacutainer tube

0-2 years: 0.5 mL Lavender top (EDTA) Microtainer
2-10 years: 2 mL Lavender top (EDTA) Vacutainer tube

Red, Gold, or Light Green (Li-heparin) top tubes are NOT acceptable.
Collection Information: Pre-chill Vacutainer, place on ice immediately after blood collection, and deliver to lab immediately.

Minimum volume of plasma required is 700 L for adult samples or 200 L for pediatric samples.
Test Schedule: Tuesday afternoons
Turnaround time from when specimen
is received in testing laboratory:
5 days
Reference Range: 7 - 10 am: 1.6 - 13.9 pmol/L
Effective Date: 2009-02-12
Revised Date: 2019-12-09
Critical Value:  
Interpretive Comments: ACTH levels undergo diurnal variation. Values are normally higher in the morning and lower in the evening.
Special Processing: Use a refrigerated centrifuge to separate plasma. Analyze samples immediately or freeze them at -20 C.
Comments: Biotin may interfere with this test. Samples should not be taken from patients receiving high biotin doses (i.e. > 5 mg/day) until at least 8 hours after the last biotin administration.
Method of Analysis: Roche Electrochemiluminescence
Critical Information Required:  
Storage & Shipment: Store and ship plasma frozen.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul