Laboratory Test Information Guide

Details for Alkaline Phosphatase Isoenzymes, Serum

Test Name: Alkaline Phosphatase Isoenzymes, Serum
Alternate Name(s): Alkaline Phosphatase Fractionation
ALP Isoenzymes
Laboratory: Clinical Immunology
Specimen Type: Adult:
5 mL Gold top Vacutainer tube

0-2 years: 0.5 mL Red or Gold top Microtainer
2-10 years: 3 mL Red top Vacutainer tube

Light Green (Li-Heparin) or Lavender (EDTA) top tubes are NOT acceptable
Collection Information: Minimum volume of serum required is 200 ÁL.
Test Schedule: Monday - Friday
Turnaround time from when specimen
is received in testing laboratory:
10 days
Reference Range: ALP Isoenzyme Fraction Reference Interval (U/L)

AgeLiver 1BoneLiver 2Intestinal
≤14 days≤77≤248≤17≤35
15 days-<1 year≤145≤469≤33≤66
1-<10 years≤104≤335≤23≤47
10-<13 years≤129≤417≤29≤58
Male 13-<15 years≤145≤468≤33≤66
Male 15-<17 years≤103≤331≤23≤46
Male 17-<19 years≤46≤149≤10≤21
Male ≥19 years≤92≤97≤12≤18
Female 13-<15 years≤79≤254≤18≤36
Female 15-<17 years≤36≤117≤8≤16
Female 17-<19 years≤27≤87≤6≤12
Female ≥19 years≤75≤77≤15≤15
Effective Date: 2010-01-11
Revised Date: 2020-05-25
Critical Value:  
Interpretive Comments: 1. The reference values of the individual isoenzymes vary with age, sex, hormonal state (pregnancy, menopause, puberty) and medications.

2. In children, ALP is increased due to predominant bone isoenzymes.

3. Intestinal isoenzymes are absent in about 60% of normal subjects

4. Our method does not differentiate Placental ALP from other bands.
Special Processing:  
Comments: If alkaline phosphatase isoenzymes are ordered and it has been ≤ 15 days since the collection date of the last sample run, the test will be cancelled. Please monitor using the total alkaline phosphatase result.
Method of Analysis: Sebia gel electrophoresis
Critical Information Required:  
Storage & Shipment: Store and ship serum frozen.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul