Laboratory Test Information Guide

Details for Amniotic Fluid

Test Name: Amniotic Fluid
Alternate Name(s): Chromosome Analysis
Laboratory: Cytogenetics (VH)
Specimen Type: 20 mL of amniotic fluid for QF-PCR, Microarray (if required) and back up culture (additional sample if external testing required)

3mL EDTA Maternal Blood Sample
Collection Information: Patients should be referred to the Genetics Clinic at LHSC, VH (Ext.58140)
Amniotic fluid should be collected in two sterile tubes and shipped STAT to the laboratory - Do not refrigerate, freeze or expose to heat.

System Codes:
CGen Pntl
Microarray (MD Microarray)
Requisition: CYTOGENETICS REQUISITION . (must include patient's name, address, Ontario Health Insurance Number, PIN (if applicable), originating location, Dr's full name and address, test requested, specimen type and pertinent clinical information.

Test Schedule: As required
Turnaround time from when specimen
is received in testing laboratory:
QF-PCR - 3 Days
Microarray or Chromosome Analysis - 2-3 weeks
Reference Range: See final report
Effective Date:  
Revised Date: 2017-11-08
Critical Value: See final report
Interpretive Comments: Prenatal diagnostic test performed at approximately 16-18 weeks gestation.
Special Processing: Must prearrange with Cytogenetics Lab.
Comments: The Cytogenetics Lab is staffed from 0700-1700 (Monday-Friday), Ext. 78974 (office), or 75714 (lab).

For additional information please refer to the Cytogenetics Webpage.
Method of Analysis:  QF-PCR (Rapid Aneuploidy Detection)
Cell culture
Chromosome Karyotyping (depending on QFPCR results)
Prenatal Microarray (depending on clinical indications)
Fluorescence in situ hybridization (FISH) (depending on microarray results)
Critical Information Required: Specimen should be taken with enough time to ensure receipt in the
laboratory by 1500 h
Storage & Shipment: If unable to transport immediately, store at 4oC and transport at room temperature (15-25oC) within 24 hours.
System Codes: 
See Collection Information
Referred To:  

Questions? Comments? Contact Laura Gopaul