Laboratory Test Information Guide

Details for Anti-AT1R

Test Name: Anti-AT1R
Alternate Name(s): Angiotensin II Receptor Subtype 1 antibody
Laboratory: Transplant
Specimen Type: 6 mL Red top Vacutainer tube - No additives or separator gel
Collection Information: Draw by venipuncture and pre-dialysis (if applicable) to avoid contamination with anticoagulants.
Test Schedule: As required Monday to Friday except Stat holidays
Turnaround time from when specimen
is received in testing laboratory:
14 days
Contact the laboratory at ext. 33320
Reference Range: <10 U/mL Negative
10-17 U/mL Borderline Positive
>17 U/mL Positive
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments: Angiotensin II is an oligopeptide hormone that causes vasoconstriction and release of aldosterone from the adrenal cortex, increasing blood pressure. AT1R antibodies have been linked to severe vascular rejection and malignant hypertension.
Special Processing:  
Comments: This test will include pre-transplant and post transplant samples to differentiate between pre-tx and de novo antibody.

It is common to hold off testing until several samples are available for testing (maximum is 14 days).
Method of Analysis: This test is performed using an EIA method (Enzyme Immuonassay)
Critical Information Required: Indicate if the patient is undergoing therapy with products or medication containing therapeutic antibodies such as Rituxan, Campath, Eculizumab, IVIG, OKT3, ALG, etc. as these may interfere with the results.

Indicate if the patient has had any sensitizing events such as blood transfusions, previous transplants, pregnancies etc. as these could influence the interpretation of the results.

Also indicate if the patient is undergoing a change in immunosuppression regime or is being treated by plasma exchange since these actions may explain fluctuations in results.
Storage & Shipment: Samples drawn at LHSC are delivered to the Transplant Laboratory at room temperature.

Samples for Anti AT1R Testing (1 red top) drawn outside of LHSC must be spun and the serum removed and frozen. Ship on dry ice if shipping requires >24 hours. Ship frozen on freezer packs if the sample will be received in < 24 hours.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul