Laboratory Test Information Guide

Details for Anti-SARS-CoV-2 Total Antibody

Test Name: Anti-SARS-CoV-2 Total Antibody
Alternate Name(s): Anti-SARS-CoV-2 Antibody
COVID Serology
Anti-SARS-CoV-2 (IgG + IgM)
Laboratory: Core
Specimen Type: Adult:
4.5 mL Green (Lithium Heparin) top Vacutainer

Serum from a 5 mL Gold top or 6 mL Red top is also acceptable.

0-2 years: 0.5 mL Green or Gold top Microtainer
2-10 years: 3 mL Green top or 2 mL Gold top tube
Collection Information: Collect blood aseptically in a Vacutainer tube.
Test Schedule: Monday - Friday
0800 - 1600 h
Turnaround time from when specimen
is received in testing laboratory:
Up to 48 hours
Reference Range: See report
Effective Date: 2020-08-01
Revised Date: 2020-01-15
Critical Value:  
Interpretive Comments: Interpretive Comments: Testing detects both IgG and IgM antibodies (total) to SARS-CoV-2 nucleocapsid (N) antigen.

Negative Results:
Negative results do not rule out acute SARS-CoV-2 infection. If acute infection is suspected, consider SARS-CoV-2 RNA testing or repeat testing in 7 to 10 days. False-negative results may occur in immunocompromised individuals or those with mild illness. Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions.

Positive Results:
Presence of SARS-CoV-2 antibodies indicates current or previous infection. False-positive results may occur due to cross-reacting antibodies or other causes. It is unknown whether antibodies indicate protective immunity.
Special Processing:  
Comments: Serology testing examines a person’s blood to see if they have antibodies to COVID-19. Testing detects both IgG and IgM antibodies (total) to SARS-CoV-2 nucleocapsid (N) antigen of the SARS-CoV-2 virus.

Serology testing is limited to the currently approved uses in Ontario (See PHO COVID-19 Serology) including:

• The investigation of suspected cases of Multisystem Inflammatory Syndrome in Children (MIS-C)
With Approval: Serology may be considered in patients with severe illness who have tested repeatedly negative by PCR and where serology results would be a helpful adjunctive tool for clinical/public health action and decision making

Serology testing should NOT be used for:
• the diagnosis of acute infection or determining if a patient is infectious
• determining immune status of the patient
Please contact Dr Michael Knauer, Clinical Biochemist (ext. 52237 or pager 17130) for questions and approval
Method of Analysis: Test performed by Roche Elecsys Anti-SARS-CoV-2 Electrochemiluminescence immunoassay targeting the nucleocapsid (N) antigen.
Critical Information Required:  
Storage & Shipment: Samples stable 7 days at 2-8 0C, freeze at -20 0C for longer storage.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul