Laboratory Test Information Guide

Details for Blood Culture – Aerobic/Anaerobic (Paediatric)

Test Name: Blood Culture – Aerobic/Anaerobic (Paediatric)
Alternate Name(s):  
Laboratory: Microbiology
Specimen Type: Blood
Collection Information: This sample type cannot be processed as a STAT request. Results are reported as they become available.

Blood cultures should be collected on each patient based on the clinical suspicion of a septic episode. The number of cultures to collect will vary between one or two separate collections within a 24-hour period.

Collection procedures:
Please refer to the blood culture collection procedures:
Blood culture collection procedure – Paediatric, Venipuncture

Blood culture collection procedure – Central Venous Access Device

Blood sampling volumes

The appropriate blood volume to collect in children is dependent on weight.

For patients weighing less than 35 kg, please refer to Blood Volume Requirements for Paediatric Blood Culture Collections

For patients weighing more than 35 kg, please refer to LTIG Blood Culture -Aerobic/Anaerobic (Adult)

Blood cultures for Candida species
No additional procedures are required for the routine isolation of Candida species from the blood.

Blood cultures for the fungal organisms Histoplasma capsulatum, and Blastomyces dermatitidis
Please refer to Laboratory Test Information Guide: Blood culture - Malassezia species, Histoplasma capsulatum, and Blastomyces dermatitidis.
Test Schedule: Daily
Turnaround time from when specimen
is received in testing laboratory:
5 days – positive findings are available within 1 hour of discovery. Negative cultures at 24 hour
Reference Range: 5 days – positive findings are available within 1 hour of discovery.

Negative cultures at 24 hours will be routinely updated with the comment “No growth 1 day”.
Effective Date: 2018-04-09
Revised Date: 2020-11-03
Critical Value:  
Interpretive Comments: Recovery of organisms depends mainly on volume of blood collected and frequency of collection. Timing is less important, particularly if cultures are collected before antimicrobial therapy.
Special Processing:  
Method of Analysis: Continuous monitoring blood culture system
Critical Information Required:  
Storage & Shipment: Transport bottle to the lab promptly; all bottles must be received within 24 hours of collection. Please see Blood culture transportation to Lab for proper transport.

If there is a delay in transport, bottles can be left at room temperature. Never refrigerate.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul