Laboratory Test Information Guide

Details for Bone Marrow Aspirate Examination

Test Name: Bone Marrow Aspirate Examination
Alternate Name(s): Bone Marrow Smears
For: a) Wright's b) Iron Stain c) Cytochemical Stains

Differential Bone Marrow
Laboratory: Flow Cytometry
Specimen Type: Bone marrow in K2 or K3 EDTA
Collection Information: Minimum volume of bone marrow required is 1 mL.

The bone marrow collection tube is provided in a kit supplied by CSR. (It is the Wards'/Units' responsibility to stock these kits).

If "touch films" of the bone marrow have been prepared at the bedside, they are to be sent with the bone marrow aspirate to the Investigational Hematology Laboratory at Victoria Hospital as well.

For referred-in (non-LHSC) samples:

1. From primary collection tube, prepare 4 push and 4 squash films. DO NOT STAIN.

2. Fix films in absolute methanol for 10 minutes.

3. Label all films with patientís name.

4. Air-dry and package in slide mailers.

5. Include the following:

∑ Requisition with patient demographics (HCN included)
∑ Relevant clinical history (reason for test request)
∑ Remainder of EDTA anticoagulated bone marrow aspirate sample
∑ CBC results
∑ Wright-Giemsa (or equivalent) peripheral blood film
∑ A fax number to which the report can be sent

6. Send to:
Flow Cytometry
Room D1-202
London Health Sciences Centre
800 Commissioners Rd, London, ON
N6A 5W9

7. Notify laboratory (519-685-8500 ext 57450) prior to sending sample.
Test Schedule: Monday-Friday
Turnaround time from when specimen
is received in testing laboratory:
2 weekdays
Reference Range: Normal marrow elements
Effective Date: 2006-06-01
Revised Date: 2019-05-17
Critical Value:  
Interpretive Comments: The bone marrow report is prepared following examination of Wrights-Giemsa and Prussian Blue stained films. CBC results and possibly special cytochemical stained films also impact the interpretation.
Special Processing: Films must be prepared ASAP after collection.
Method of Analysis: Microscopy
Critical Information Required: -Clinical history
-Reason for test request
Storage & Shipment: Ship sample at room temperature.

Samples collected within LHSC/SJH must be shipped within 30 minutes of collection and received for testing within 3 hours.

Referred in samples must be shipped within 30 minutes of collection and received for testing within 8 hours.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul