Laboratory Test Information Guide

Details for C3 Nephritic Factor

Test Name: C3 Nephritic Factor
Alternate Name(s): C3NF
Laboratory: Clinical Immunology
Specimen Type: 6 mL Red top Vacutainer tube. Gel barrier tubes are not acceptable.

0-2 yrs: Red 0.5pk.
2-10 yrs: 2 mL Red top
Collection Information: N.B: This test is an out-of-country request. Pre-approval by the Immunology section head (Dr. L. Yang ext. 35768), Monday-Friday 0800-1600 hrs is required prior to collection.

Record collection time.

Send the sample to the laboratory ASAP.

Minimum serum volume is 0.5 mL
Test Schedule: Referred out monthly
Turnaround time from when specimen
is received in testing laboratory:
40 days
Reference Range: Normal Ratio 0.00 to 0.26
Equivocal Ratio 0.27 to 0.33
Positive Ratio greater than or equal to 0.34
Effective Date: 2010-01-11
Revised Date: 2020-05-20
Critical Value:  
Interpretive Comments: C3 Nephritic Factor is an autoantibody that binds to the alternative pathway C3 convertase and prolongs its activity in vivo.
Special Processing: Allow the filled red top tube to clot at 22-37oC for 30-60 minutes. Centrifuge at room temperature. Aliquot into 2 storage tubes and freeze as soon as possible. Store at -20oC (preferably -70oC).
Comments: This test is available exclusively to SJHC/LHSC physicians.
Method of Analysis: Immunofixation Electrophoresis
Critical Information Required: LHSC (SRA to Immunology Lab)- ship frozen on ice packs. Avoid freeze/thaw cycles.
Storage & Shipment: LHSC (SRA to Immunology Laboratory) ship frozen on ice packs. Avoid freeze/thaw cycles.

Frozen at -70C: 1 year
System Codes: 
Referred To: Referred to:

National Jewish Medical and Research Center
1400 Jackson St.,
Denver, Colorado

Questions? Comments? Contact Laura Gopaul