Laboratory Test Information Guide

Details for C9,Serum

Test Name: C9,Serum
Alternate Name(s): C9 complement component level
Laboratory: Clinical Immunology
Specimen Type: 5 mL Gold top Vacutainer tube or 6 mL Red top Vacutainer tube and a 5 mL Lavender top (EDTA plasma)

Pediatric:
0-2 yrs: Red 0.5pk. and Purple 0.5 pk.
2-10 yrs: 2 mL Red and Purple top
Collection Information: Both plasma and serum are required.
Request CH50 (TCOM) on the same sample.
Record collection time.
Mix the lavender tube well after procurement.
Send the sample to the laboratory ASAP.
Minimum serum volume is 0.5 mL per aliquot.
Requisition: GENERAL LABORATORY REQUISITION
Test Schedule: Referred out monthly.
Total complement CH50 is performed as a screen to determine the component assay is required.
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
40 days
Stat
N/A
 
Reference Range: 33-95 mg/L
Effective Date: 2010-01-11
Revised Date:  
Critical Value:  
Interpretive Comments: Also included in group test CCOMPG (includes, C1qrs, C5, C6, C7, C8, C9).

Total complement (TCOM, CH50) is a screen for classical pathway function.

The component assay is a quantitative evaluation of the concentration of each component but does not evaluate the functional activity of the component.
Special Processing:  Centrifuge lavender tube (plasma) at room temperature as soon as possible after collection. Aliquot the plasma into 2 storage tubes and freeze as rapidly as possible.
Allow the filled red top tube to clot at 22-37oC for 30-60 minutes. Centrifuge at room temperature. Aliquot into storage tube and freeze as soon as possible. Store at -20oC (preferably -70o).

Aliquots:
For CH50-1 Aliquot (serum)
For C9-2 Aliquots (plasma)
Comments: This test is available exclusively to SJHC/LHSC physicians.

Total complement is assayed first to determine if the components evaluation is necessary. Only if the total complement is less than the lower limit of normal will the complement component be processed. Otherwise the component(s) is cancelled with the Comment: "Test not indicated."
Method of Analysis: RID
Critical Information Required:  
Storage & Shipment: LHSC (SRA to Immunology Laboratory) ship frozen on ice packs. Avoid freeze/thaw cycles.
System Codes: 
Cerner
C9
 
Referred To: Referred to:

National Jewish Medical and Research Center
1400 Jackson St.,
Denver, Colorado
80206

Questions? Comments? Contact Laura Gopaul