Laboratory Test Information Guide

Details for Estradiol, Plasma/Serum

Test Name: Estradiol, Plasma/Serum
Alternate Name(s): 17 Beta Estradiol
Major Estrogen
Laboratory: Core
Specimen Type: Adult:
4.5 mL Light Green top (Li-Heparin) Vacutainer tube

0-2 years: 0.5 mL Light Green top (Li-Heparin) Microtainer
2-10 years: 3 mL Light Green top (Li-Heparin) Vacutainer tube

Red, Gold, or Lavender (EDTA) top tubes are also acceptable
Collection Information: Minimum volume of plasma or serum required is 700 ÁL for adult samples or 200 ÁL for pediatric samples.
Test Schedule: As required
Turnaround time from when specimen
is received in testing laboratory:
4 hours
Reference Range: Male > 18 years: ≤ 159 pmol/L

Follicular phase: ≤ 854 pmol/L
Ovulatory phase: 151 - 1461 pmol/L
Luteal phase: ≤ 1251 pmol/L
First trimester of pregnancy: 563 - 11902 pmol/L
Second trimester of pregnancy: 5729 - 78098 pmol/L
Third trimester of pregnancy: 31287 - >110100 pmol/L
Post-menopausal: ≤ 505 pmol/L
Effective Date: 2015-06-01
Revised Date: 2018-03-06
Critical Value:  
Interpretive Comments: Estradiol can be used as an indicator of follicular function and provides information in the diagnosis of amenorrhea and infertility. During pregnancy estradiol levels increase.

In males, estradiol can be used to evaluate feminizing syndromes.
Special Processing:  
Comments: Biotin, fulvestrant (Faslodex), and steroid drugs may interfere with this test. Samples should not be taken from patients receiving high biotin doses (i.e. > 5 mg/day) until at least 8 hours after the last biotin administration.
Method of Analysis: Roche Electrochemiluminescence
Critical Information Required:  
Storage & Shipment:  
System Codes: 
Referred To:  

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