Laboratory Test Information Guide

Details for FISH Studies

Test Name: FISH Studies
Alternate Name(s): Fluorescent In Situ Hybridization
Laboratory: Cytogenetics (VH)
Specimen Type: Blood:
3-6 mL peripheral venous blood in a sterile, Sodium Heparin Vacutainer. If <3 mL is collected, it must be in a 3 mL Vacutainer to allow for appropriate sample to anticoagulant ratio.
Bone Marrow:
1-2 mL of bone marrow in a 3 mL Sodium Heparin Vacutainer (dark green top tube)
Lymph Node/Tumor:
2-3 mm2 Lymph Node or Tumor Biopsy in RPMI media (see Comments)
Paraffin Slides:
4µm sections mounted on positively charged slides, prepared and sent from a clinical pathology laboratory. A representative H&E Histology section outlining areas of interest for examination must be included with 4 paraffin slides for each FISH probe requested.
Collection Information: All specimens should be:
• at room temperature (15-25ēC),
• sterile
• sent to the laboratory within 24 hours
• Do not freeze, heat, open or separate sample (must maintain sterility).
• Lithium heparin or EDTA specimens are unacceptable.
• On requisition, please specify chromosome disease/syndrome and/or DNA probe to be tested.
Requisition: CYTOGENETICS REQUISITION (must include patient's name, address, Ontario Health Insurance Number, PIN (if applicable), originating location, Dr's full name and address, test requested (including probe), specimen type and pertinent clinical information.
Test Schedule: As required
Turnaround time from when specimen
is received in testing laboratory:
2 weeks
3 week days
Reference Range: See final report
Effective Date:  
Revised Date: 2017-11-08
Critical Value: See final report
Interpretive Comments: N/A
Special Processing: Solution for Specimen Collection:
Contact the Cytogenetics Laboratory in advance for sterile aliquots of RPMI media for lymphomas/tumors for collection. This media may be frozen, and thawed at room temperature (15-25ēC) as needed. Media must be used by the expiry date written on the tube
Comments: The Cytogenetics Lab is staffed from 0700-1700 (Monday-Friday), Ext. 78974 (office), or 78975 (lab).

For additional information please refer to the Molecular Diagnostic Laboratory
Method of Analysis: FISH
Critical Information Required:  
Storage & Shipment: If unable to transport immediately, store at 4C and transport at room temperature (15-25oC) within 24 hours.
System Codes: 
CGENRB – Specify FISH probe
Referred To:  

Questions? Comments? Contact Laura Gopaul