Laboratory Test Information Guide

Details for Glucose-6-Phosphate Dehydrogenase Assay

Test Name: Glucose-6-Phosphate Dehydrogenase Assay
Alternate Name(s): G6PD Assay
G-6-PD Assay
Laboratory: Core
Specimen Type: 4 mL K2 or K3 EDTA Lavender top Vacutainer tube

0-2 years: 0.5 mL Lavender top Microtainer
2-10 years: 3 mL Lavender top tube
Collection Information: Minimum volume required is 0.5 mL

For referred-in (non-LHSC) samples:
1. Include the following:
Requisition with patient demographics (HCN included)
Relevant clinical history (reason for test request)

2. Send to:
Core Laboratory
Room D1-218
London Health Sciences Centre
800 Commissioners Rd.,
London , ON
N6A 5W9

3. Notify laboratory (519-685-8500 ext. 52544) prior to sending sample.

A hemoglobin value is required in order to calculate the G-6-PD activity. It is important also, for interpretation purposes, to determine the percentage of reticulocytes. Therefore, a CBC and a reticulocyte count are included in the care set.
Test Schedule: Monday-Friday
Turnaround time from when specimen
is received in testing laboratory:
24 hours
Reference Range: 8.3-17.0 IU/g Hb
Effective Date: 2008-11-15
Revised Date: 2019-05-17
Critical Value: Intermediate Deficiency: 2.0-8.2 IU/g Hb
Severe Deficiency: <2.0 IU/g Hb
Interpretive Comments: Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells. Patients with G6PD deficiency are at risk of hemolytic anemia in states of oxidative stress triggered by viral or bacterial infections, antimalarial drugs (primaquine) or exposure to certain foods (fava beans).
Special Processing: All test requests for the G-6-PD assay will include a CBC and reticulocyte enumeration.
Comments: Reticulocytes have higher G-6-PDH levels than mature red cells. It is not recommended that assays be performed after a severe hemolytic crisis, since G-6-PDH levels may appear falsely elevated."

Leukocytosis falsely elevates G-6-PDH levels in extreme anemia.
Method of Analysis: Enzymatic rate method on chemistry analyzer.
Critical Information Required:  
Storage & Shipment: Ship sample at room temperature.

Samples must be received in laboratory for testing within 24 hours of sample collection.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul