Laboratory Test Information Guide

Details for HLA Assessment-Liver Recipient

Test Name: HLA Assessment-Liver Recipient
Alternate Name(s): HLA Liver Workup
Laboratory: Transplant
Specimen Type: 2 x 4 mL Lavender (EDTA) top Vacutainer tubes

1 x 6mL Red top Vacutainer tube - No additives or separator gel
Collection Information: HLA Recipient Assessments are performed only for patients in the London Transplant Program.
Requisition: TRANSPLANT LABORATORY REQUISITION
Test Schedule:  
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
Samples are stored in the laboratory until testing is indicated-see Comments:
Stat
 
 
Reference Range: cPRAs: 0-100%

HLA typing: see report
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments: Anti-HLA antibodies indicate potential sensitization to potential organ donors and some blood products.
Special Processing:  
Comments: Patients that are being assessed for placement on the LHSC liver waiting list should have this test ordered. Antibody testing and HLA typing will be performed only when the recipient is activated on the waiting list.
Method of Analysis: cPRA determinations are determined with the TGLN or CBS cPRA calculator and are based on specificities detected with solid phase bead technology using the Luminex® platform.
HLA Class I and Class II typings are performed using molecular techniques (RSSO or SSP).
Critical Information Required: Indicate if the patient is undergoing therapy with products or medication containing therapeutic antibodies such as Rituxan, Campath, Eculizumab, IVIG, OKT3, ALG, etc. as these may interfere with the results.

Indicate if the patient has had any sensitizing events such as blood transfusions, previous transplants, pregnancies etc. as these could influence the interpretation of the results.

Also indicate if the patient is undergoing a change in immunosuppression regime or is being treated by plasma exchange since these actions may explain fluctuations in results.
Storage & Shipment: Samples drawn @ LHSC are delivered to the Transplant Laboratory at room temperature.

Samples for Antibody Testing (1 red top) drawn outside of LHSC must be spun and the serum removed and frozen. Ship on dry ice if shipping requires >24 hours. Ship frozen on freezer packs if the sample will be received in < 24 hours.

Samples for HLA Typing (2 Lavender tops) drawn outside of LHSC must be shipped at room temperature
Extracted DNA samples and sera will be stored in the laboratory, should further testing be required.
System Codes: 
Cerner
HLA Assessment-Liver Recipient
 
Referred To:  

Questions? Comments? Contact Laura Gopaul