Laboratory Test Information Guide

Details for HLA B27 Typing

Test Name: HLA B27 Typing
Alternate Name(s): HLA B27 Tissue Typing
Laboratory: Transplant (UH)
Specimen Type: 4 x 6 mL Green (Sodium Heparin) top Vacutainer tube without gel separator
This is the preferred specimen.
-Order HLA B27 Serology (B27)


2 x 4mL Lavender (EDTA) top Vacutainer tubes
-Use when leukocyte irregularities are possible or if the transit time is expected to exceed 24 hours
-Order HLAB27 Special Order (SSP)
Collection Information: This test is only available to patients from LHSC or SJHC.

Friday draws should be avoided, if possible. Draw the samples (4 x 6 mL Green NaHep tubes) early in the morning if a Friday draw is unavoidable and order HLAB27 Serology (B27).

When a later Friday draw is absolutely necessary, draw 2 x 4 mL Lavender EDTA tubes and order HLA B27 Special Order (SSP).
Test Schedule: As required
Monday to Friday
Turnaround time from when specimen
is received in testing laboratory:
7-21 days
Reference Range: See report
Effective Date: 2006-07-07
Revised Date: 2012-10-15
Critical Value: N/A
Interpretive Comments: HLA B27 Positive indicates that the patient expresses HLA B27 antigen on his/her cells.

HLA B27 Negative indicates that the patient does not express HLA B27 antigen on his/her cells.
Special Processing:  
Method of Analysis: Microlymphocytotoxicity or PCR-SSP
Critical Information Required:  
Storage & Shipment: Keep blood at room temperature and deliver within 24 hours of collection.
System Codes: 
see Specimen Type & Collection
Referred To:  

Questions? Comments? Contact Laura Gopaul