Laboratory Test Information Guide

Details for HLA BoneMarrow/StemCell Histocompatibility-Donor

Test Name: HLA BoneMarrow/StemCell Histocompatibility-Donor
Alternate Name(s): BoneMarrow/StemCell Donor initial HLA Typing
Laboratory: Transplant
Specimen Type: 2 x 4 mL Lavender (EDTA) top Vacutainer tubes

Note: Increase collection volumes if leukocyte count is low, smaller volumes apply for paediatric patients.

Paediatric Patients:
<1 year: 2 mL EDTA
1-10 years: 2 mL EDTA
10-18 years: 4 mL EDTA

Contact the laboratory if these volumes are not possible: 519-663-3320
Collection Information: BM/SC transplant workups are coordinated by the Bone Marrow Transplant Coordinator who can be contacted via the LHSC switchboard (519-658-8500).

Potential donor testing is usually restricted to close relatives. Contact the laboratory or the BM/SC coordinators for details.

Testing is only performed for patients involved in the London Transplant Program. Unrelated marrow / stem cell donors are not normally tested in this laboratory. Please contact the laboratory (519-663-3320) if other situations apply to your patient.
Requisition: TRANSPLANT LABORATORY REQUISITION
Test Schedule: As required Monday to Friday except Stat holidays
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
14 days
Stat
1-3 days if blood is received in the laboratory before 0900
 
Reference Range:  
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments:  
Special Processing:  
Comments:  
Method of Analysis: Molecular PCR-RSSO, RT-PCR typing kits are available to provide generic (low to intermediate) level HLA typing. PCR-SSP kits will usually be used for high resolution typings.
Critical Information Required: The Recipient ID (MRN#) and relationship to the recipient is required information.
Storage & Shipment: Samples drawn @ LHSC are delivered to the Transplant Laboratory at room temperature.

Samples for HLA Typing (2 Lavender tops) drawn outside of LHSC must be shipped at room temperature and arrive within 72 hrs.

Extracted DNA samples will be stored in the laboratory, should further testing be required.
System Codes: 
Cerner
HLA BM/SC Donor Case
 
Referred To:  

Questions? Comments? Contact Laura Gopaul