Laboratory Test Information Guide

Details for HLA BoneMarrow/StemCell Histocompatibility-Recipient

Test Name: HLA BoneMarrow/StemCell Histocompatibility-Recipient
Alternate Name(s): BoneMarrow/StemCell recipient initial HLA Typing
Laboratory: Transplant
Specimen Type: 4 x 4 mL Lavender (EDTA) top Vacutainer tubes

1 x 6 mL Red top Vacutainer tube - No additives or separator gel

Note: Increase collection volumes if leukocyte count is low, smaller volumes apply for paediatric patients.

Paediatric Patients:
<1 year: 2 mL EDTA + 2 mL clotted
1-10 years: 2 mL EDTA + 6 mL clotted
10-18 years: 4 mL EDTA + 6 mL clotted

Contact the laboratory if these volumes are not possible: 519-663-3320

Chemotherapy and some drugs may interfere with WBC number and/or morphology. Samples should be drawn prior to treatment whenever possible.
Collection Information: BM/SC transplant workups are coordinated by the Bone Marrow Transplant Coordinator who can be contacted via the LHSC switchboard (519-658-8500)

Testing is only performed for patients involved in the London Transplant Program. Unrelated marrow / stem cell donors are not normally tested in this laboratory. Please contact the laboratory (519-663-3320) if other situations apply to your patient.
Test Schedule: As required Monday to Friday except Stat holidays
Turnaround time from when specimen
is received in testing laboratory:
14 days
2-3 days if blood is received in the laboratory before 0900
Reference Range: See report
Effective Date: 2020-06-29
Revised Date: 2020-08-24
Critical Value:  
Interpretive Comments:  
Special Processing:  
Method of Analysis: Molecular PCR-RSSO, RT-PCR typing kits are available to provide generic (low to intermediate) level HLA typing. PCR-SSP kits will usually be used for the high resolution typings.
Critical Information Required:  
Storage & Shipment: Samples drawn @ LHSC are delivered to the Transplant Laboratory at room temperature.

Samples for HLA Typing (4 Lavender tops) drawn outside of LHSC must be shipped at room temperature and arrive within 72 hrs.

Extracted DNA samples and the serum sample will be stored in the laboratory, should further testing be required.
System Codes: 
HLA BM/SC Recipient Case
Referred To:  

Questions? Comments? Contact Laura Gopaul