Laboratory Test Information Guide

Details for HLA Confirmatory Typing- Recipient

Test Name: HLA Confirmatory Typing- Recipient
Alternate Name(s):  
Laboratory: Transplant
Specimen Type: 4 x 4 mL Lavender (EDTA) top Vacutainer tubes

Note: Increase collection volumes if leukocyte count is low, smaller volumes apply for paediatric patients.

Paediatric Patients:
<1 year: 2 mL EDTA
1-10 years: 2 mL EDTA
10-18 years: 4 mL EDTA

Contact the laboratory if these volumes are not possible: 519-663-3320
Collection Information: This sample is collected at a time and date that is
different from the initial HLA typing. It is usually
drawn about 1 week prior to the transplant.

The results must match those obtained from the
original sample.

Chemotherapy and some drugs may interfere with
WBC number and/or morphology. Samples should be drawn prior to treatment whenever possible.
Test Schedule: As required Monday to Friday except Stat holidays
Turnaround time from when specimen
is received in testing laboratory:
1-3 days
1-3 days if blood is received in the laboratory before 0900
Reference Range:  
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments: A low resolution HLA Class I typing will be performed. The results must match the original HLA typing obtained on the previous sample.
Special Processing:  
Comments: BM/SC transplant workups are coordinated by the Bone Marrow Transplant Coordinator who can be contacted via the LHSC switchboard (519-658-8500)

Confirmatory HLA typings should be performed at the centre that is to do the transplant. Therefore, some testing for the paediatric program may be sent to the Toronto program.
Method of Analysis: Molecular PCR-RSSO, RT-PCR typing kits are available to provide generic (low to intermediate) level HLA typing.
Critical Information Required:  
Storage & Shipment: Samples drawn @ LHSC are delivered to the Transplant Laboratory at room temperature.

Samples for HLA Typing (4 Lavender tops) drawn outside of LHSC must be shipped at room temperature and arrive within 72 hrs.

Extracted DNA samples will be stored in the laboratory, should further testing be required.
System Codes: 
HLA ConfirmatoryType-Recipient
Referred To:  

Questions? Comments? Contact Laura Gopaul