Laboratory Test Information Guide

Details for HLA Donor Specific Antibody (DSA testing)

Test Name: HLA Donor Specific Antibody (DSA testing)
Alternate Name(s): DSA testing
Laboratory: Transplant (UH)
Specimen Type: 6 mL Red top Vacutainer tube
Collection Information: Draw by venipuncture and pre-dialysis (if applicable) to avoid contamination with anticoagulants.

Requisition: HLA Antibody Investigation (NS5920)
Test Schedule: Testing is performed Mondays, Wednesdays and Fridays except on Statutory holidays.
Turnaround time from when specimen
is received in testing laboratory:
7 days
Same day if the sample is received in the Transplant Laboratory before 0830, MWF-except Stats
Reference Range:  
Effective Date: 2011-03-15
Revised Date: 2012-10-15
Critical Value:  
Interpretive Comments: Antibodies directed against donor HLA antigens may predict risk of rejection.
Special Processing: Draw by venipuncture and pre-dialysis (if applicable) to avoid contamination with anticoagulants.
Comments: This test should only be ordered at the request of the medical team when following a transplanted patient in a London solid organ transplant program.

Contact the Transplant Laboratory to discuss other situations. (519-663-3320)
Method of Analysis: Solid phase bead technology using the Luminex® platform.
Critical Information Required: Indicate if the patient is undergoing therapy with products or medication containing human antibodies such as OKT3, IVIG, ALG etc. as this may interfere with the results. Also indicate if the patient is undergoing a change in immunosuppression regime or is being treated by plasma exchange since these actions may explain fluctuations in results.

DSA analysis requires knowledge of the HLA typing of the donor or prospective donor of interest. Patients who were not transplanted in the London program will need to have this information forwarded to this laboratory.
Storage & Shipment: Samples that are to be received within 24 hours are to be stored and shipped refrigerated. Samples that are delayed beyond 24 hours are to have the serum separated, frozen and shipped on dry ice (or frozen ice packs, if dry ice is not available).
System Codes: 
HLA Donor Specific AntibodyIgG
Referred To:  

Questions? Comments? Contact Laura Gopaul