Laboratory Test Information Guide

Details for HLA Donor Specific Antibody (DSA)

Test Name: HLA Donor Specific Antibody (DSA)
Alternate Name(s): 
Laboratory: Transplant
Specimen Type: 6 mL Red top Vacutainer tube - No additives or separator gel
Collection Information: Draw pre-dialysis (if applicable) and by venipuncture to avoid contamination with anticoagulants.
Test Schedule: Monday to Friday except on Stat holidays
Turnaround time from when specimen
is received in testing laboratory:
1-3 days
Same day if blood is received in the laboratory before 0900
Reference Range: DSA detected or not detected
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments: Antibodies directed against donor HLA antigens may predict a risk of rejection
Special Processing:  
Comments: This test should only be ordered when following a patient in a London transplant program.

Contact the Transplant Laboratory to discuss other situations. (519-663-3320)
Method of Analysis: Solid bead technology using the Luminex® platform
Critical Information Required: Critical Information Required Indicate if the patient is undergoing therapy with products or medication containing therapeutic antibodies such as Rituxan, Campath, Eculizumab, IVIG, OKT3, ALG, etc. as these may interfere with the results.

Indicate if the patient has had any sensitizing events such as blood transfusions, previous transplants, pregnancies etc. as these could influence the interpretation of the results.

Also indicate if the patient is undergoing a change in immunosuppression regime or is being treated by plasma exchange since these actions may explain fluctuations in results.

DSA analysis requires knowledge of the HLA typing of the donor or prospective donor of interest. Patients who were not transplanted in the London program will need to have this information forwarded to this laboratory.
Storage & Shipment: Samples drawn at LHSC are delivered to the Transplant Laboratory at room temperature.

Samples for Antibody Testing (1 red top) drawn outside of LHSC must be spun and the serum removed and frozen. Ship on dry ice if shipping requires >24 hours. Ship frozen on freezer packs if the sample will be received in < 24 hours.
System Codes: 
HLA DSA IgG:HLA Donor Specific
Referred To:  

Questions? Comments? Contact Laura Gopaul