Laboratory Test Information Guide

Details for HLA Freeze PBL

Test Name: HLA Freeze PBL
Alternate Name(s): Freeze Blood
Laboratory: Transplant
Specimen Type: Freeze Cells from a patient or donor at times other than pre-op.

Donor Requirements:
6 x 6 mL Sodium Heparin (dark green tops, no separator gel)

Note: smaller volumes apply for paediatric patients. Contact the Transplant Laboratory for details: 519-663-3320
Collection Information: Living donor crossmatches are performed only for patients in the London Transplant Program and must be booked in advance by calling the laboratory. (519-663-3320)

The patients are usually bled at the UH LTC, OP clinics or on the floor. Samples are delivered to the Transplant Laboratory @room temperature.

Samples that are being shipped into the laboratory should be drawn and shipped @ room temperature.
Test Schedule: As required Monday to Friday except Stat holidays
Turnaround time from when specimen
is received in testing laboratory:
Reference Range:  
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments:  
Special Processing:  
Comments: Living donor transplant workups, including the freezing of donor cells are coordinated by the Living Donor Transplant Coordinator who can be contacted via the LHSC switchboard (519-658-8500)
Method of Analysis: Donor lymphocytes are isolated and frozen in liquid nitrogen.
Critical Information Required: The recipient ID (MRN#) is required when ordering HLA Freeze PBL on a living donor. There is a prompt in Cerner when the order is placed.
Storage & Shipment: Frozen cells will be stored in the laboratory, should future testing be required.
System Codes: 
HLA Freeze PBL
Referred To:  

Questions? Comments? Contact Laura Gopaul