Laboratory Test Information Guide

Details for HLA Pre-op Recipient

Test Name: HLA Pre-op Recipient
Alternate Name(s): 
Laboratory: Transplant (UH)
Specimen Type: 6 mL Red top Vacutainer tube - No additives or separator gel
Collection Information: Draw pre-dialysis (if applicable) and by venipuncture to avoid contamination with anticoagulants.
Drawn upon pre-op admission to LHSC in preparation for transplant
Test Schedule: This sample may be used in the prospective/retrospective flow crossmatch and updated SAB testing may be performed.

All samples will be archived for possible testing at a later date.
Turnaround time from when specimen
is received in testing laboratory:
1-3 days
Same day if blood is received in the laboratory before 0900
Reference Range: cPRAs: 0-100% (HLA Antibody Testing)

DSA detected or not detected
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments: Anti-HLA antibodies indicate sensitization to potential organ donors and some blood products.
Antibodies directed against donor HLA antigens may predict a risk of rejection
Special Processing:  
Comments: Pre-op patients that are being prepared for transplant at LHSC (Kidney, Kidney/Pancreas, Pancreas, Liver/Kidney, or Heart) should have this test ordered. Liver patients: see “HLA Pre-op Liver”
The sample may be used for cPRA, crossmatching, DSA testing or it may be stored for future testing as outlined in the current program-specific policies.
Method of Analysis: cPRA determinations are obtained by using the TGLN or CBS cPRA calculator and are based on specificities detected with solid phase bead technology using the Luminex® platform.
Critical Information Required: Indicate if the patient is undergoing therapy with products or medication containing therapeutic antibodies such as Rituxan, Campath, Eculizumab, IVIG, OKT3, ALG, etc. as these may interfere with the results.

Indicate if the patient has had any sensitizing events such as blood transfusions, previous transplants, pregnancies etc. as these could influence the interpretation of the results.

Also indicate if the patient is undergoing a change in immunosuppression regime or is being treated by plasma exchange since these actions may explain fluctuations in results.
Storage & Shipment: Samples drawn at LHSC are delivered to the Transplant Laboratory at room temperature.

Pre-op serum samples will be stored in the laboratory, should further testing be required.
System Codes: 
HLA Pre-op Recipient
Referred To:  

Questions? Comments? Contact Laura Gopaul