Laboratory Test Information Guide

Details for HLA Serum for Crossmatch LD-Recipient

Test Name: HLA Serum for Crossmatch LD-Recipient
Alternate Name(s): 
Laboratory: Transplant
Specimen Type: 6 mL Red top Vacutainer tube - No additives or separator gel
Collection Information: Draw pre-dialysis (if applicable) and by venipuncture to avoid contamination with anticoagulants.
Test Schedule: Monday to Friday except on Stat holidays

This test should be ordered to be drawn on the same day the final crossmatch against a living donor is to be performed.
Turnaround time from when specimen
is received in testing laboratory:
14 days
Reference Range: Crossmatch Positive or Negative
cPRAs: 0-100% (HLA Antibody Testing)
DSA detected or not detected
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments: A positive crossmatch may indicate the presence of anti-donor antibodies.

Anti-HLA antibodies indicate sensitization to potential organ donors and some blood products.
Antibodies directed against donor HLA antigens may predict a risk of rejection
Special Processing:  
Comments: This sample will be one of the samples used for a flow cytometer crossmatch against a living donor.

In addition the sample may be used for cPRA or DSA testing as outlined in the program-specific polices.
Method of Analysis: cPRA determinations are obtained by using the TGLN or CBS cPRA calculator and are based on specificities detected with solid phase bead technology using the Luminex® platform.
Living donor crossmatches are performed using flow cytometry.
Critical Information Required: Indicate if the patient is undergoing therapy with products or medication containing therapeutic antibodies such as Rituxan, Campath, Eculizumab, IVIG, OKT3, ALG, etc. as these may interfere with the results.

Indicate if the patient has had any sensitizing events such as blood transfusions, previous transplants, pregnancies etc. as these could influence the interpretation of the results.

Also indicate if the patient is undergoing a change in immunosuppression regime or is being treated by plasma exchange since these actions may explain fluctuations in results.

DSA analysis requires knowledge of the HLA typing of the donor or prospective donor of interest. Patients who were not transplanted in the London program will need to have this information forwarded to this laboratory.
Storage & Shipment: Samples drawn at LHSC are delivered to the Transplant Laboratory at room temperature.
Samples for Antibody Testing (1 red top) drawn outside of LHSC must be spun and the serum removed and frozen. Ship on dry ice if shipping requires >24 hours. Ship frozen on freezer packs if the sample will be received in < 24 hours.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul