Laboratory Test Information Guide

Details for HLA Transfusion Refractory

Test Name: HLA Transfusion Refractory
Alternate Name(s):  
Laboratory: Transplant
Specimen Type: 6 mL Red top Vacutainer tube - No additives or separator gel
Collection Information: Draw pre-dialysis (if applicable) and by venipuncture to avoid contamination with anticoagulants.
Test Schedule: Monday to Friday
Turnaround time from when specimen
is received in testing laboratory:
1-3 days
Same day if blood is received in the laboratory before 0900
Reference Range: Antibodies detected or not detected

Any HLA antibodies that were detected will be reported in the comment field in Cerner as Weak, Moderate and Strong based on MFI values.
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments: Anti-HLA antibodies indicate sensitization to potential donors and some blood products
Special Processing:  
Comments: This test is ordered for recipient antibody identification in preparation for receiving HLA matched platelets
Method of Analysis: Solid bead technology using the Luminex® platform
Critical Information Required: HLA typing of patient

Indicate if the patient is undergoing therapy with products or medication containing therapeutic antibodies such as Rituxan, Campath, Eculizumab, IVIG, OKT3, ALG, etc. as these may interfere with the results.
Indicate if the patient has had any sensitizing events such as blood transfusions, previous transplants, pregnancies etc. as these could influence the interpretation of the results.

Also indicate if the patient is undergoing a change in immunosuppression regime or is being treated by plasma exchange since these actions may explain fluctuations in results.
Storage & Shipment: Samples drawn at LHSC are delivered to the Transplant Laboratory at room temperature.
Samples for Antibody Testing (1 red top) drawn outside of LHSC must be spun and the serum removed and frozen. Ship on dry ice if shipping requires >24 hours. Ship frozen on freezer packs if the sample will be received in < 24 hours.
System Codes: 
HLA Transfusion Refractory
Referred To:  

Questions? Comments? Contact Laura Gopaul