Laboratory Test Information Guide

Details for HLA Workup Living Donor Additional or Final

Test Name: HLA Workup Living Donor Additional or Final
Alternate Name(s): Additional Kidney Living donor Crossmatch
Final Kidney Living donor Crossmatch
Laboratory: Transplant
Specimen Type: Donor Requirements:
14 x 6mL Sodium Heparin (dark green tops, no separator gel)

Note: smaller volumes apply for paediatric patients. Contact the Transplant Laboratory for details: 519-663-3320
Collection Information: Living donor crossmatches are performed only for patients in the London Transplant Program and must be booked in advance by calling the laboratory. (519-663-3320)
It is preferable to have the living donors bled at the UH LTC. However, shipped samples may be accepted in rare cases.
Test Schedule: As required Monday to Friday except Stat holidays
Turnaround time from when specimen
is received in testing laboratory:
7 days
1-3 days
Reference Range: Living Donor Crossmatch: Negative / Borderline / Positive
Effective Date: 2020-06-29
Revised Date:  
Critical Value:  
Interpretive Comments: The cPRA is an indicator of how broadly any patientís HLA antibody reacts to random donors. Elevated cPRAs may reflect a higher level of difficulty in finding an acceptable donor.
A positive flow cytometry crossmatch indicates the likely presence of donor specific antibodies.
Special Processing:  
Comments: Living donor transplant workups are coordinated by the Living Donor Transplant Coordinator who can be contacted via the LHSC switchboard (519-658-8500).
Method of Analysis: Crossmatches are performed using flow cytometry.

Excess cells will be frozen in liquid nitrogen for possible future testing.
Critical Information Required: The recipient ID (MRN#) is required when ordering the testing.
Storage & Shipment: Living donor samples are usually drawn in the Lab Test Centre (UH) and delivered immediately to the Transplant Laboratory at room temperature. Shipped living donor samples must be kept at room temperature and delivered within 24 hours.
System Codes: 
HLA Workup Living Additional
Referred To:  

Questions? Comments? Contact Laura Gopaul