Laboratory Test Information Guide

Details for Hereditary Spherocytosis Screening Test

Test Name: Hereditary Spherocytosis Screening Test
Alternate Name(s):  
Laboratory: Flow Cytometry
Specimen Type: 4 mL K2 or K3 EDTA Lavender top Vacutainer tube

0-2 years: 0.5 mL Lavender Microtainer
2-10 years: 3 mL Lavender top tube
Collection Information: Minimum volume required is 1.0 mL

Collect sample in time for testing by noon on Friday.

For referred-in (non-LHSC) samples:

1. Include the following:

Requisition with patient demographics (HCN included)
Relevant clinical history (reason for test request)
CBC results
A fax number to which the report can be sent

2. Send to:

Flow Cytometry
Room D1-202
London Health Sciences Centre
800 Commissioners Rd, London, ON
N6A 5W9

3. Notify the laboratory (519-685-8500 ext. 57450) prior to sending sample.
Test Schedule: Monday - Thursday
0800 - 1500
0800 - 1200
Turnaround time from when specimen
is received in testing laboratory:
24 hours
4 hours
Reference Range: Negative for Hereditary Spherocytosis
Effective Date: 2007-11-01
Revised Date: 2019-06-05
Critical Value:  
Interpretive Comments: Negative:

The screening test is negative for Hereditary Spherocytosis. The method is highly predictive for HS, however, may not be reliable in post-splenectomy patients or in Hereditary Spherocytosis due to an ankyrin deficiency.


The screening test is positive for Hereditary Spherocytosis.
Special Processing:  
Method of Analysis: Flow Cytometry
Critical Information Required:  
Storage & Shipment: Ship sample at 4C prior to testing (Do not freeze).

Sample is stable for 72 hours at 4C.

Samples are to be shipped within 4 hours of collection.

Samples must be received for testing within 12 hours of collection.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul