Laboratory Test Information Guide

Details for Herpes Simplex Virus PCR Herpes simplex Type 1/Type 2 HSVPCR

Test Name: Herpes Simplex Virus PCR Herpes simplex Type 1/Type 2 HSVPCR
Alternate Name(s):  
Laboratory: Virology Laboratory
Specimen Type: CSF
Plasma - EDTA (Lavender top Vacutainer tube)
Swabs - lesions
Tissue
Fluids
Collection Information: Blood (EDTA): MUST be spun, separated and stored at 4C for shipment the next morning if there is a delay of ≥4 hours to the Microbiology laboratory

CSF and Fluid: Collect a minimum of 1.0 mL in a sterile container.

Lesion: Wipe vesicle with saline. Disrupt vesicle and collect fluid and cells from base of lesion with a swab.

Tissue: Place in sterile container

In General: Swabs contained in the Viral Transport Kit should be used for collection.

Viral Transport Media with swab can be ordered from HMMS using item # 36981.
Requisition: VIROLOGY REQUISITION
Test Schedule: CSF samples are tested once daily Monday to Friday.
Blood and lesions are tested three times a week on Monday, Wednesday and Friday.
Tissues and Fluids are sent to Public Health Laboratory for testing
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
Blood and Lesions: 3-5 days
Stat
CSF: 72 hours
 
Reference Range: See report
Effective Date: 2011-10-20
Revised Date: 2019-10-07
Critical Value:  
Interpretive Comments: Sample analyzed for Herpes simplex type 1 and 2 and Varicella Zoster Virus
Special Processing: For SRA:

After 9 pm: Centrifuge and aliquot plasma. Refrigerate and send to Microbiology the following morning
Comments:  
Method of Analysis: PCR (polymerase chain reaction)
Critical Information Required: Patient symptoms
Storage & Shipment: Blood (EDTA): MUST be spun, separated and stored at 4C for shipment the next morning if there is a delay of ≥4 hours to the Microbiology laboratory

All other specimen types: must be received within 24 hours of collection. If the transport time exceeds 24 hours, the specimen will only be tested if appropriate.
System Codes: 
Cerner
HSVPCR / VZVPCR
 
Referred To:  

Questions? Comments? Contact Laura Gopaul