Laboratory Test Information Guide

Details for Mycoplasma/Ureaplasma Culture

Test Name: Mycoplasma/Ureaplasma Culture
Alternate Name(s): Ureaplasma/Mycoplasma Culture
Laboratory: Microbiology (VH)
Specimen Type: 
Male (Adult):
Urine (1mL minimum)

Female (Adult):
Products of Conception
Vaginal lesion
Amniotic fluid (1 mL minimum)
Urine (1 mL minimum)

Endotracheal suction tube transported in Virus/Chlamydia/Mycoplasma transport media.
Collection Information: Note: Use sterile cotton, dacron or rayon-tipped swabs with plastic or aluminum shafts. Do not use calcium alginate swabs and avoid wooden-shafted swabs. Express material from swabs into Virus/Chlamydia/Mycoplasma (VCM) transport media and discard swab.

Urethra - Express and discard any exudate. Insert flexible, fine-shafted swab 2-4 cm into urethra, gently rotate swab 2-3 times to absorb sample.

Cervix - Remove exocervical mucous with swab and discard swab. Insert fresh swab at least 1 cm into cervical canal and rotate swab 2-3 times to absorb sample.

Vaginal lesion - Swab back and forth over lesion.

Fluids - Add between 0.5 and 1 mL of specimen to Virus/Chlamydia/Mycoplasma (VCM) transport media.

Urine - Collect first morning, midstream, clean void in a sterile container.

Endotracheal suction tube - Place in a sterile container and add Virus/Chlamydia/Mycoplasma (VCM) transport media.
Test Schedule: Referred out weekdays to the Public Health Laboratory.
Turnaround time from when specimen
is received in testing laboratory:
14 days
Reference Range:  
Effective Date: 2010-09-13
Revised Date: 2018-06-15
Critical Value:  
Interpretive Comments:  
Special Processing: 2-8oC if shipped within 48 hours
Freeze at -70oC and ship on ice packs if >48 hours
Comments: Faulty collection and delay in transport of specimens are the primary causes of test failure.
Method of Analysis: Culture
Critical Information Required:  
Storage & Shipment: The specimen must be refrigerated.
If stored >48 hours, freeze at -70oC.
The specimen must be received within 24 hours of collection in the Microbiology Laboratory.
System Codes: 
Referred To: Public Health Laboratory

Questions? Comments? Contact Laura Gopaul