Laboratory Test Information Guide

Details for Neisserria gonorrhoeae Nucleic Acid Amplification Test

Test Name: Neisserria gonorrhoeae Nucleic Acid Amplification Test
Alternate Name(s):  
Laboratory: Microbiology (VH)
Specimen Type: -Cervix or Urethra Swab

Collection Information: Please use the single Unisex swab for collecting cervix and urethral swabs. Urine samples, collected in a sterile container, will have to be transferred into a collection kit specific for NGCAD testing. Please view the information on collection requirements using these new kits here.

Collection Requirements:

Cervix or Urethra Swab:

Use Genprobe Aptima® Swab Specimen Transport Tube (White label and lid).

Females - Using the large swab (white handle), remove excess mucous from the exocervix and discard. Collect sample from cervix using the small swab (blue handle) and place sample swab into white transport tube.

Males - Collect sample from urethra using the small swab (blue handle) and place swab into white transport tube.


Use Genprobe Aptima® Urine Collection Tube (Yellow label and white lid).
(Also used for patients who have had a hysterectomy). Indicate on the requisition-"hysterectomy patient."

First morning void (20-30 mL)into a sterile container. Transfer urine into transport tube. Fill tube only to the Fill Area indicated by arrows. Do NOT over fill or under fill as sample will be unsuitable for testing.

Test Schedule: Referred out weekdays to Public Health Laboratory.
Turnaround time from when specimen
is received in testing laboratory:
3 days
Reference Range:  
Effective Date: 2010-09-13
Revised Date: 2018-06-15
Critical Value:  
Interpretive Comments: Paediatric and medical - legal assault cases and test of cure situations must be investigated by culture technique.

However, nucleic acid amplification tests (NAATs) may be acceptable if positive results are confirmed by a second set of primers. If available, both tests (culture and NAAT) should be taken. Molecular diagnostic tests, especially NAATs are more sensitive than culture. Genprobe Aptima® Assay confirmatory testing is available for both Chlamydia trachomatis and Neisseria gonorrhoeae.
Special Processing: Same specimen can be tested for Chlamydia.
Comments: Faulty collection and delays in transport of specimen are the primary causes of test failure.
Method of Analysis: Nucleic Acid Amplification (NAAT)
Critical Information Required:  
Storage & Shipment: Samples must be stored at room temperature or refrigerated. DO NOT FREEZE.

The specimen must be received within 24 hours of collection.

Do NOT break foil on top of cap.
System Codes: 
Referred To: Public Health Laboratory

Questions? Comments? Contact Laura Gopaul