Laboratory Test Information Guide

Details for Paraneoplastic Antibody Panel

Test Name: Paraneoplastic Antibody Panel
Alternate Name(s): Panel includes: anti-Hu (ANNA-1); anti-Yo (PCA-1 or Purkinje cell
antibody-1), anti-Ri (ANNA-2), anti-CV2 (anti-CRMP5),
anti-Ma2/Ta (PNMA2) and anti-Amphiphysin (Anti-AMPH)
Laboratory: Clinical Immunology
Specimen Type: Serum preferred
5 mL Gold top or 6 mL Red top Vacutainer tube
Samples collected in EDTA, heparin or citrate will be accepted
CSF samples will also be accepted.
Note: CSF samples will only be processed if accompanied by a serum sample
Collection Information: Require 0.5 mL of serum or plasma or CSF (accompanied by a serum or plasma sample).
Test Schedule: Batched once every two weeks.
Turnaround time from when specimen
is received in testing laboratory:
15 business days
Reference Range: Negative
Effective Date: 2015-04-01
Revised Date: 2017-03-17
Critical Value:  
Interpretive Comments: Paraneoplastic syndromes are a group of rare disorders; some of which are caused by autoimmune reactions against antigens co-expressed by cancer cells and neurons. Below are the most frequent cancers associated with paraneoplastic syndromes.

Antibody Neurological DisorderAssociated Tumour
Anti-HuEncephalomyelits, Sensory neuropathySmall cell lung cancer, Neuroblastoma
Anti-YoCerebellar degenerationOvarian cancer, Breast cancers
Anti-RiAtaxia with or without Opsoclonus-myclonusBreast cancer, Small cell lung cancer
Anti-Ma2/TaBrain stem encephalitis, Limbic encephalitisTesticular cancer
Anti-AmphiphysinStiff person syndromeBreast cancer, Small cell lung cancer
Anti-CV2Limbic encephalomyelitisSmall cell lung cancer, Thymoma

Special Processing:  
Method of Analysis: Two different methods, immunofluorescence assay and Immunoblot, are used to detect paraneoplastic antibodies.

The use of a substrate combination of primate cerebellum and primate
intestine permits the secure differentiation of various antibodies with
neuronal specificity from other autoantibodies in the same test procedure.

A second method, immunoblot is performed to confirm findings.
Critical Information Required: CSF orders will be canceled if a serum is not received with the sample.
Storage & Shipment: Samples may be stored up to 14 days refrigerated (2C to 8C). For longer storage freeze at -20C.
System Codes: 
PNACSF (CSF & serum)
Referred To:  

Questions? Comments? Contact Laura Gopaul