Laboratory Test Information Guide

Details for Paraneoplastic Antibody Panel

Test Name: Paraneoplastic Antibody Panel
Alternate Name(s): Panel includes:
Anti-Hu (ANNA-1)
Anti-Yo (PCA-1 or Purkinje cell antibody-1)
Anti-Ri (ANNA-2)
Anti-CV2 (anti-CRMP5)
Anti-Ma2/Ta (PNMA2)
Anti-Amphiphysin (Anti-AMPH)
Anti-Tr (DNER)
Laboratory: Clinical Immunology
Specimen Type: 6 mL Red or 5 mL Gold top Vacutainer tube
EDTA, heparin or citrate plasma are also acceptable
CSF samples will also be accepted
Collection Information: Minimum volume of serum or plasma or CSF (accompanied by a serum sample) is 0.5 mL
Test Schedule: Batched analysis
Turnaround time from when specimen
is received in testing laboratory:
15 days
Reference Range: Negative
Effective Date: 2015-04-01
Revised Date: 2019-10-08
Critical Value:  
Interpretive Comments: Paraneoplastic syndromes are a group of rare disorders; some of which are caused by autoimmune reactions against antigens co-expressed by cancer cells and neurons. Below are the most frequent cancers associated with paraneoplastic syndromes and autoantibodies.

Antibody Neurological DisorderAssociated Tumour
Anti-HuEncephalomyelits, Sensory neuropathySmall cell lung cancer, Neuroblastoma
Anti-YoCerebellar degenerationOvarian cancer, Breast cancers
Anti-RiAtaxia with or without Opsoclonus-myclonusBreast cancer, Small cell lung cancer
Anti-Ma2/TaBrain stem encephalitis, Limbic encephalitisTesticular cancer
Anti-AmphiphysinStiff person syndromeBreast cancer, Small cell lung cancer
Anti-CV2Limbic encephalomyelitisSmall cell lung cancer, Thymoma
Anti-recoverinRetinopathySmall cell lung cancer
Anti-SOX1Lambert-Eaton myasthenic syndrome, Cerebellar degenerationSmall cell lung cancer
Anti-titinMyasthenia gravisThymoma
Anti-zic4Cerebellar degenerationSmall cell lung cancer
Anti-GAD65Stiff person syndromeBreast carcinoma, Colon carcinoma
Anti-Tr (DNER)Cerebellar degenerationHodgkin's lymphoma

Special Processing: Testing is available to the LHSC/SJH physicians and will also be accepted from outside sources.
Comments: Immunoblot for Paraneoplastic antibodies is not suitable for the detection of antibodies against GAD65 in diabetes mellitus. A negative result does not exclude the presence of Anti-GAD65.
Method of Analysis: Two different methods, immunofluorescence assay and immunoblot, are used to detect paraneoplastic antibodies.

The use of a substrate combination of primate cerebellum and primate
intestine permits the secure differentiation of various antibodies with
neuronal specificity from other autoantibodies in the same test procedure.

A second method, immunoblot is performed to confirm findings. Anti-titin and anti-recoverin antibodies will be determined by immunoblot only.
Critical Information Required:  
Storage & Shipment: Samples may be stored up to 14 days refrigerated (2C to 8C). For longer storage freeze at -20C.
System Codes: 
PNACSF (CSF & serum)
Referred To:  

Questions? Comments? Contact Laura Gopaul