Laboratory Test Information Guide

Details for Paraneoplastic Antibody Panel

Test Name: Paraneoplastic Antibody Panel
Alternate Name(s): Panel includes:
Anti-Hu (ANNA-1)
Anti-Yo (PCA-1 or Purkinje cell antibody-1)
Anti-Ri (ANNA-2)
Anti-CV2 (anti-CRMP5)
Anti-Ma2/Ta (PNMA2)
Anti-Amphiphysin (Anti-AMPH)
Anti-Tr (DNER)
Laboratory: Clinical Immunology
Specimen Type: 6 mL Red or 5 mL Gold top Vacutainer tube
EDTA, heparin or citrate plasma are also acceptable
CSF samples will also be accepted. Suggest CSF be submitted with serum for testing. Serum is more sensitive than CSF for Paraneoplastic antibodies.
Collection Information: Minimum volume of serum or plasma or CSF is 0.5 mL
Test Schedule: Batched analysis
Turnaround time from when specimen
is received in testing laboratory:
15 days
Reference Range: Negative
Effective Date: 2015-04-01
Revised Date: 2020-05-25
Critical Value:  
Interpretive Comments: Paraneoplastic syndromes are a group of rare disorders; some of which are caused by autoimmune reactions against antigens co-expressed by cancer cells and neurons. Below are the most frequent cancers associated with paraneoplastic syndromes and autoantibodies.

Antibody Neurological DisorderAssociated Tumour
Anti-HuEncephalomyelits, Sensory neuropathySmall cell lung cancer, Neuroblastoma
Anti-YoCerebellar degenerationOvarian cancer, Breast cancers
Anti-RiAtaxia with or without Opsoclonus-myclonusBreast cancer, Small cell lung cancer
Anti-Ma2/TaBrain stem encephalitis, Limbic encephalitisTesticular cancer
Anti-AmphiphysinStiff person syndromeBreast cancer, Small cell lung cancer
Anti-CV2Limbic encephalomyelitisSmall cell lung cancer, Thymoma
Anti-recoverinRetinopathySmall cell lung cancer
Anti-SOX1Lambert-Eaton myasthenic syndrome, Cerebellar degenerationSmall cell lung cancer
Anti-titinMyasthenia gravisThymoma
Anti-zic4Cerebellar degenerationSmall cell lung cancer
Anti-GAD65Stiff person syndromeBreast carcinoma, Colon carcinoma
Anti-Tr (DNER)Cerebellar degenerationHodgkin's lymphoma

Special Processing: Testing is available to the LHSC/SJH physicians and will also be accepted from outside sources.
Comments: Immunoblot for Paraneoplastic antibodies is not suitable for the detection of antibodies against GAD65 in diabetes mellitus. A negative result does not exclude the presence of Anti-GAD65.
Method of Analysis: Two different methods, immunofluorescence assay and immunoblot, are used to detect paraneoplastic antibodies.

The use of a substrate combination of primate cerebellum and primate
intestine permits the secure differentiation of various antibodies with
neuronal specificity from other autoantibodies in the same test procedure.

A second method, immunoblot is performed to confirm findings. Anti-titin, anti-SOX-1 and anti-recoverin antibodies will be determined by immunoblot only.
Critical Information Required:  
Storage & Shipment: Samples may be stored up to 14 days refrigerated (2C to 8C). For longer storage freeze at -20C.
System Codes: 
PNAS for serum/PNACSF for CSF
Referred To:  

Questions? Comments? Contact Laura Gopaul