Laboratory Test Information Guide

Details for Paroxysmal Nocturnal Hemoglobinuria Investigation

Test Name: Paroxysmal Nocturnal Hemoglobinuria Investigation
Alternate Name(s):  
Laboratory: Flow Cytometry (VH)
Specimen Type: Peripheral blood collected in a 4 mL K2 or K3 EDTA Lavender top Vacutainer tube
Collection Information: Minimum volume required is 1.5 mL.
Requisition: Referred-In Samples: FLOW CYTOMETRY REQUISITION
Test Schedule: Monday-Friday

Fridays or prior to STAT holidays, routine specimens must be received in the Flow Cytometry Laboratory at Victoria Hospital by 1300.
Turnaround time from when specimen
is received in testing laboratory:
48 hours
Reference Range: No PNH clones are a normal result.
Effective Date: 2006-06-01
Revised Date: 2021-03-05
Critical Value:  
Interpretive Comments: Negative result is normal expression of GPI linked proteins.

Positive result is a percent of GPI linked proteins are absent from RBCs and/or WBCs.
Special Processing:  
Comments: Test is positive in Paroxysmal Nocturnal Hemoglobinuria (PNH) and HEMPAS.

Call Flow Cytometry Lab (519) 685-8500 x 57450 for further details.
Method of Analysis: In vitro analysis for detection of GPI-anchored membrane proteins on leukocytes and erythrocytes using Flow Cytometry
Critical Information Required:  
Storage & Shipment: Specimens are to be shipped/transported within 1 hour of collection to the Flow Cytometry Laboratory at Victoria Hospital at room temperature.

Samples must be received and processed within 72 hours of collection by the Flow Cytometry Laboratory.

Once received by the Flow Cytometry Laboratory, samples are to be stored at 4oC.
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul