Laboratory Test Information Guide

Details for Respiratory Virus Panel (RPCR)

Test Name: Respiratory Virus Panel (RPCR)
Alternate Name(s): Influenza Virus
Flu Screen

Laboratory: Virology Laboratory
Specimen Type: Nasopharyngeal Aspirate
Nasopharyngeal Swab
Bronchial Alveolar Lavage (BAL)
Collection Information: Collection Information: Respiratory virus PCR (RPCR) includes the detection of Influenza A, Influenza B, Respiratory Syncytial Virus (RSV),from a single specimen.

Testing eligibility includes:
- Hospital patient settings including in-patient and emergency department encounters
- Outbreak investigations at the direction of the Public Health Unit

COVID-19 Assessment Centre and other community-based encounters will continue to be eligible for COVID-19 testing only

Nasal/Nasopharyngeal swab-
Insert flexible, fine-shafted swab into nostril or nasopharynx, rotate in circular manner to collect cellular material. Place swab in viral transport media.

Nasopharyngeal Aspirate- Using mucous collection device, insert appropriately sized catheter nasally into posterior nasopharynx. Apply suction, using intermittent suction as catheter is withdrawn. If necessary use small amount of viral transport media to wash sample out of tubing. Transfer aspirated sample to a sterile container.

Bronchoalveolar Lavage- Collect a minimum volume of 1 mL of BAL.
Transfer sample to a sterile container.
Test Schedule: From November 16 to April 12, specimens will be processed for Influenza A, Influenza B, RSV, and SARS CoV-2 (COVID-19) 7 days a week. No further testing will be performed on specimens from ER or outpatients unless requested by the clinician.

Specimens from adult patients admitted to ICU, all BAL and all pediatric patient samples (inpatient and ICU) that are negative for Influenza A, Influenza B, RSV, will be tested for Parainfluenza 1, 2, 3, 4, Human Adenovirus, Human Metapneumovirus and Human Enterovirus (Includes EVD68). Additional testing will be performed by special request from the clinician.

From May 1 to November 15, samples will be processed Monday, Wednesday and Friday only. Testing will be restricted to patients from Critical Care Units. A full viral panel will be tested which includes Influenza A, Influenza B, RSV A/B, Parainfluenza 1, 2, 3, 4, Adenovirus, Metapneumovirus, Enterovirus, Bocavirus, Seasonal Coronaviruses and Rhinovirus. Testing on any other patients will require consultation with the Microbiologist on call.
Turnaround time from when specimen
is received in testing laboratory:
May 1-Nov. 15:
4 days
Nov. 16-Apr. 30:
24 hours
Reference Range: See report
Effective Date: 2010-08-01
Revised Date: 2020-11-16
Critical Value:  
Interpretive Comments:  
Special Processing:  
Method of Analysis: PCR (polymerase chain reaction)
Critical Information Required:  
Storage & Shipment: Specimen must be received within 24 hours of collection. If the transport time exceeds 24 hours, the specimen will only be tested if appropriate
System Codes: 
Referred To:  

Questions? Comments? Contact Laura Gopaul