Laboratory Test Information Guide

Details for T-Cell Lymphoma

Test Name: T-Cell Lymphoma
Alternate Name(s): TCRG Gene clonality assay
Laboratory: Molecular Diagnostics
Specimen Type: EDTA blood/bone marrow
Collection Information: Send directly to lab at room temperature
Test Schedule: As required,
Monday to Friday 0800-1600h
Turnaround time from when specimen
is received in testing laboratory:
15 days
Reference Range: See report
Effective Date:  
Revised Date: 2021-05-14
Critical Value: N/A
Interpretive Comments: PCR-based assays are used for the identification of T-cell and B-cell clonal populations. Each B- and T-cell has a single productive V-J rearrangement that is unique in both length and sequence. Therefore, when this region is amplified using flanking DNA, a clonal population of cells yields one or two prominent amplified products within the expected size range. In addition to lymphoid neoplasms, clonal/oligoclonal populations may be seen in reactive/inflammatory conditions, autoimmune disease, immunodeficiency/immunosuppression, and transplant patients, among other entities. Clonal patterns that may be due to an exaggerated/abnormal T-cell repertoire/response should be interpreted with caution. Correlation with clinical, flow cytometric and histological features (if applicable) is essential.
Special Processing:  
Method of Analysis: Based on the BIOMED-2 strategy (genomic DNA is amplified by multiplex PCR using a commercial kit (InVivoScribe Technologies) containing fluorescently labeled primers that target either conserved variable, diversity, and joining regions flanking the unique hypervariable antigen
Critical Information Required:  
Storage & Shipment: Deliver to lab at room temperature.
System Codes: 
T-Cell Lymphoma
Referred To:  

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