Laboratory Test Information Guide

Details for Virus Culture

Test Name: Virus Culture
Alternate Name(s):  
Laboratory: Virology Laboratory
Specimen Type: Specimen should reflect the target organism whenever possible.

Respiratory Viruses:
See RPCR Respiratory Virus Panel

Herpes Simplex Virus:
See HSV PCR


Varciella zoster viruses:
VZVPCR

Adenovirus:
swabs - conjunctiva, rectal, stools

MUMPS:
Swabs-throat, buccal
Urine
CSF

MEASLES:
Swabs-nasopharyngeal, conjunctival, throat
CSF
Urine
Autopsy material

Rubella:
Swabs-throat, nasopharyngeal
Urine
Aborted material

Enterovirus:
stools (all other specimens see ENVPCR)

Viral Transport Media with swab can be ordered from HMMS using item # 36981
Collection Information: Fluids (CSF, urine):
Collect a minimum of 1 mL in a sterile container.

Gastrointestinal:
Rectal - insert swab 4-6 cm into rectum and roll swab against the mucosa.
Stool - Place 10-15 mL in a sterile container.


Ocular:
Conjunctival - Swab lower conjunctiva with flexible, fine-shafted swab.
Scrapings - Conjunctiva - Scrape the lower tarsal area with a sterilized spatula.
Cornea - Use short, firm strokes in one direction and scrape multiple areas of ulceration and suppuration. Keep the eyelid open and be careful not to touch the eyelashes. Place scrapings in viral transport media.

Throat:
Vigorously swab tonsillar areas and posterior nasopharynx. Use tongue blade to depress tongue to prevent contamination with saliva.

In General:
Swabs used must be sterile cotton, dacron or rayon with plastic or aluminum shafts. Do not use calcium alginate swabs and avoid swabs with wooden shafts.

Place swabs, scrapings and small pieces of tissue into a small volume of virus transport media.

Place bulk specimens into sterile containers; add a small amount of viral transport media to prevent drying of tissue. Do not add viral transport media to urines, sterile fluids or stools.

Viral Transport Media with Swab can be ordered from HMMS using item # 36981.
Requisition: PUBLIC HEALTH LABORATORY TEST REQUISITION
Test Schedule: Referred out weekdays to Public Health Laboratory
Turnaround time from when specimen
is received in testing laboratory:
 
Routine
Sample dependent
Stat
 
 
Reference Range: See report
Effective Date: 2010-08-09
Revised Date: 2018-06-15
Critical Value:  
Interpretive Comments:  
Special Processing:  
Comments:  
Method of Analysis: Shell vial culture
Critical Information Required:  
Storage & Shipment: Specimen must be received within 24 hours of collection. If the transport time exceeds 24 hours, the specimen will only be tested if appropriate.
System Codes: 
Cerner
VIRCRO
 
Referred To: Central Public Health Lab, Toronto

Questions? Comments? Contact Laura Gopaul